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Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering
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Date
2018-01-01
Author
Pekozer, Gorke Gurel
Ramazanoglu, Mustafa
Schlegel, Karl Andreas
Kök, Fatma Neşe
KÖSE, GAMZE
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Stem cells of dental origin emerged as a new source for the regeneration of tissues with advantages mainly including non-invasive collection procedures and lack of ethical contraversies with their harvest or use. In this study, porcine TGSCs (pTGSCs) were isolated from mandibular third molar tooth germs of 6-month-old domestic pigs. This is the first study that reports the isolation and characterization of TGSCs from porcine third molars and their differentiation depending on STRO-1 expression. PTGSCs were sorted according to their STRO-1 expression as STRO-1(+) and STRO-1(-). Sorted and unsorted heterogenous cells (US) were characterized by their osteogenic, chondrogenic and adipogenic differentiation capabilities. STRO-1(+) cells exhibited a higher proliferation rate owing to their clonogenic properties. All three groups of cells were found differentiated into osteogenic lineage as shown by ALP activity, calcium deposition assay, detection of osteogenic mRNAs and, proteins and mineralization staining. According to differentiation analysis, STRO-1(+) cells did not show a better performance for osteogenesis compared to STRO-1(-) and US cells. This might indicate that STRO-1(+) cells might require a heterogeneous population of cells including STRO-1(-) in their niche to perform their proposed role in osteogenesis.
Subject Keywords
Biotechnology
,
Medicine (miscellaneous)
,
Pharmaceutical Science
,
Biomedical Engineering
,
General Medicine
URI
https://hdl.handle.net/11511/67906
Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
DOI
https://doi.org/10.1080/21691401.2017.1332637
Collections
Department of Biology, Article