Molecular detection of Bacillus anthracis: evaluation of the efficiency of DNA extraction and a novel dry PCR

Ortatatli, Mesut
Kenar, Levent
Sezigen, Sermet
Eyison, Koray
Oktem, Huseyin
Background: Due to recent increase in mailings of anthrax spores, the detection of bioweapons has gained a great deal of interest. This study aimed to investigate the yield and purity of DNA obtained from spores and vegetative forms of Bacillus anthracis for detection by conventional (wet) and dry (lyophilized) PCR methods.


Robust background normalization method for one-channel microarrays
AKAL, TÜLAY; Purutçuoğlu Gazi, Vilda; Weber, Gerhard-Wilhelm (Walter de Gruyter GmbH, 2017-04-01)
Background: Microarray technology, aims to measure the amount of changes in transcripted messages for each gene by RNA via quantifying the colour intensity on the arrays. But due to the different experimental conditions, these measurements can include both systematic and random erroneous signals. For this reason, we present a novel gene expression index, called multi-RGX (Multiple-probe Robust Gene Expression Index) for one-channel microarrays.
Aromatic amino acid synthesis performance of bacillus acidocaldarius
Kocabaş, Pınar; Çalık, Pınar; Department of Chemical Engineering (2004)
In this study, the effects of bioprocess operation parameters on aromatic amino acid synthesis performance of Bacillus acidocaldarius were investigated. Firstly, in laboratory scale shake-bioreactors, a defined medium was designed in terms of its carbon and nitrogen sources, to achieve the highest cell concentration. Thereafter, the effects of bioprocess operation parameters, i.e., pH and temperature were investigated; and the optimum medium contained (kg m-3): fructose, 8; (NH4)2HPO4, 5; CaCl2, 0.2; KH2PO4...
Hplc-dad isolation of antioxidant compounds in aesculus hippocastanum bark extracts and cytotoxic effects on hl-60 cells
Özdoğan, Nizamettin; Özdoğan, Nizamettin; Department of Biochemistry (2007)
This study was designed to investigate the cytotoxic and antioxidative properties of Aesculus hippocastanum L. (A. hippocastanum) bark extracts. Dried and powdered barks were extracted in ethanol, methanol, water and ethylacetate at a ratio of 1:6 (w/v). Antioxidative capacity of each extract (ethanol, methanol, water and ethylacetate) were determined by their ability to scavenge 1, 1 -diphenyl-2-picryl-hydrazyl radical (DPPH). Effective concentration (EC50) values were calculated as 0.010 mg/mL 0.011 mg/mL...
Immunotherapeutic applications of CpG ODN
Gürsel, Mayda (2006-06-01)
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate the mammalian immune system to mount a rapid innate immune response. This response is characterized by the production of polyreactive IgM, immunomodulatory cytokines and chemokines. CpG ODN directly stimulate lymphocytes, natural killer cells and professional antigen-presenting cells (such as macrophages and dendritic cells). Owing to the strength and nature of this stimulation, CpG ODN are being harnessed f...
Examination of the dielectrophoretic spectra of MCF7 breast cancer cells and leukocytes
Çağlayan, Zeynep; Demircan Yalçın, Yağmur; Külah, Haluk (Wiley, 2020-03-01)
The detection of circulating tumor cells (CTCs) in blood is crucial to assess metastatic progression and to guide therapy. Dielectrophoresis (DEP) is a powerful cell surface marker-free method that allows intrinsic dielectric properties of suspended cells to be exploited for CTC enrichment/isolation from blood. Design of a successful DEP-based CTC enrichment/isolation system requires that the DEP response of the targeted particles should accurately be known. This paper presents a DEP spectrum method to inve...
Citation Formats
M. Ortatatli, L. Kenar, S. Sezigen, K. Eyison, and H. Oktem, “Molecular detection of Bacillus anthracis: evaluation of the efficiency of DNA extraction and a novel dry PCR,” TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, pp. 147–152, 2019, Accessed: 00, 2020. [Online]. Available: