Alterations in zinc status and tissue structures of heparin-induced osteoporotic rabbits

Date

2004-01-01

Author

Turan, B
Zaloglu, N
Saran, Y
Konukseven, E
Koc, E

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

134
views

0
downloads

Heparin can cause some complications in tissues when it is used for long-term therapy. Besides its beneficial effects, it may have some side effects. In this study, we investigated the possible hematological and histopathological changes in rabbit tissues under long-term (1 month) and overdoses (1,000 and 2,000 IU/kg/day) heparin applications. Group 1 was injected with serum physiologic and kept as control while Groups 2 and 3 were injected with heparin as dosages of 1,000 IU/kg/day and 2,000 IU/kg/ day, respectively. There were no significant differences between the average values of serum electrolytes and copper levels and liver enzymes ALT, AST of 2 experimental groups and the control group while their serum zinc levels (4.54 +/- 0.52 mug/ml and 4.72 +/- 0.65 mug/ml) were significantly (p < 0.05) lower than the controls (5.75 +/- 0.72 mug/ml). Hemoglobin level of Group 3 was significantly lower than of Group 2 and the control. In light microscopy, investigation of liver tissues of Groups 2 and 3, we observed mainly some disturbances in vascular outflow, disruption of normal histologic organization resulting in loss of cord architecture, intralobular inflammation with disruption of cords. Focal necrotic areas with eosinophilic amorphous materials in aortic tissue and edema, enlarged perivascular areas and interstitial tissue with scattered lymphocyte in myocardium were observed in Group 2. Degenerative regions with amorphous materials and calcification like atheroma plaque in aorta, some necrotic cells in myocardium were observed in Group 3. In lung tissues from Groups 2 and 3, interstitial imflammation, widened and densely infiltrated interalveolar septa and necrotic cells in the alveoles were seen. Alveoli were also dilated and filled with edema fluid. These findings suggest that long-term and overdose heparin application have degenerative side effects on tissues and especially on serum zinc level.

Subject Keywords

Inorganic Chemistry, Clinical Biochemistry, Biochemistry

URI

https://hdl.handle.net/11511/68181

Journal

TRACE ELEMENTS AND ELECTROLYTES

DOI

https://doi.org/10.5414/tep21033

Collections

Unclassified, Article

Suggestions

OpenMETU
Core

Examination of the dielectrophoretic spectra of MCF7 breast cancer cells and leukocytes Çağlayan, Zeynep; Demircan Yalçın, Yağmur; Külah, Haluk (Wiley, 2020-03-01) The detection of circulating tumor cells (CTCs) in blood is crucial to assess metastatic progression and to guide therapy. Dielectrophoresis (DEP) is a powerful cell surface marker-free method that allows intrinsic dielectric properties of suspended cells to be exploited for CTC enrichment/isolation from blood. Design of a successful DEP-based CTC enrichment/isolation system requires that the DEP response of the targeted particles should accurately be known. This paper presents a DEP spectrum method to inve...
Enzyme-catalyzed reductive activation of anticancer drugs ıdarubicin and mitomycin c Çelik, Haydar; Arınç, Emel; Department of Biochemistry (2008) Idarubicin (IDA) and mitomycin C (MC) are clinically effective quinone-containing anticancer agents used in the treatment of several human cancers. Quinone-containing anticancer drugs have the potential to undergo bioreduction by oxidoreductases to reactive species, and thereby exert their cytotoxic effects. In the present study, we investigated, for the first time, the potential of IDA, in comparison to MC, to undergo reductive activation by NADPH-cytochrome P450 reductase (P450R), NADH-cytochrome b5 reduc...
Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients Özcan Kabasakal, Süreyya; Vieira, Ana C.; Park, Gun Wook; Kim, Jae Han; Mannis, Mark J.; Lebrilla, Carlito B. (American Chemical Society (ACS), 2013-03-01) O-Glycans in saliva and tear isolated from patients suffering from ocular rosacea, a form of inflammatory ocular surface disease, were profiled, and their structures were elucidated using high resolution mass spectrometry. We have previously shown that certain structures, particularly sulfated oligosaccharides, increased in the tear and saliva of rosacea patients In this study, the structures of these glycans were elucidated using primarily tandem mass spectrometry. There were important similarities in the ...
The expression of gst genes in diabetic rat liver tissues İrtem Kartal, Deniz; Güray, Tülin; Department of Biochemistry (2008) Free radicals which have critical roles in living systems through their beneficial and detrimental effects play an important role in medical revolution in health. Radicals are produced in the cells and tissues of our bodies by various processes and reactions. Diabetes mellitus is an extremely common disease in the world which seems to be accompanied by a shortage of antioxidants and an increase in free radicals, the end result of oxidative stress. Glutathione S-Transferases (GST; EC 2.5.1.18) are found in e...
The serum immunoglobulin G glycosylation signature of gastric cancer Ruhaak, L. Renee; Barkauskas, Donald A.; Torres, Javier; Cooke, Cara L.; Wu, Lauren D.; Stroble, Carol; Özcan Kabasakal, Süreyya; Williams, Cynthia C.; Camorlinga, Margarita; Rocke, David M.; Lebrilla, Carlito B.; Solnick, Jay V. (Elsevier BV, 2015-03-01) Biomarkers may facilitate detection of gastric cancer at an earlier stage and reduce mortality. Here we sought to determine if the glycosylation profile of serum immunoglobulin G (IgG) could distinguish patients with non-atrophic gastritis (NAG), duodenal ulcer (DU) and gastric cancer (GC). Serum IgG was released and analyzed using nano-LC–TOF mass spectrometry. Statistically significant false discovery rate (FDR)-adjusted p-values were observed for 18 glycans, eight that differed significantly between NAG ...

Citation Formats

B. Turan, N. Zaloglu, Y. Saran, E. Konukseven, and E. Koc, “Alterations in zinc status and tissue structures of heparin-induced osteoporotic rabbits,” TRACE ELEMENTS AND ELECTROLYTES, pp. 33–40, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68181.