Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting

Boghossian, Ardemis A.
Sen, Fatih
Gibbons, Brenna M.
Sen, Selda
Faltermeier, Sean M.
Giraldo, Juan Pablo
Zhang, Cathy T.
Zhang, Jingqing
Heller, Daniel A.
Strano, Michael S.
The chloroplast contains densely stacked arrays of light-harvesting proteins that harness solar energy with theoretical maximum glucose conversion efficiencies approaching 12%. Few studies have explored isolated chloroplasts as a renewable, abundant, and low cost source for solar energy harvesting. One impediment is that photoactive proteins within the chloroplast become photodamaged due to reactive oxygen species (ROS) generation. In vivo, chloroplasts reduce photodegradation by applying a self-repair cycle that dynamically replaces photodamaged components; outside the cell, ROS-induced photodegradation contributes to limited chloroplast stability. The incorporation of chloroplasts into synthetic, light-harvesting devices will require regenerative ROS scavenging mechanisms to prolong photoactivity. Herein, we study ROS generation within isolated chloroplasts extracted from Spinacia oleracea directly interfaced with nanoparticle antioxidants, including dextran-wrapped nanoceria (dNC) previously demonstrated as a potent ROS scavenger. We quantitatively examine the effect of dNC, along with cerium ions, fullerenol, and DNA-wrapped single-walled carbon nanotubes (SWCNTs), on the ROS generation of isolated chloroplasts using the oxidative dyes, 2',7'- dichlorodihydrofluorescein diacetate (H2DCF-DA) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT). Electrochemical measurements confirm that chloroplasts processed from free solution can generate power under illumination. We find dNC to be the most effective of these agents for decreasing oxidizing species and superoxide concentrations whilst preserving chloroplast photoactivity at concentrations below 5 M, offering a promising mechanism for maintaining regenerative chloroplast photoactivity for light-harvesting applications.


Modeling, transient simulations and parametric studies of parabolic trough collectors with thermal energy storage
Akba, Tufan; Baker, Derek Keıth; Güvenç Yazıcıoğlu, Almıla (Elsevier BV, 2020-3-15)
For investigating the system response of parabolic trough collector heat generating system, a plant with parabolic trough collector field and two-tank molten salt thermal energy storage model with component-level control algorithm is developed for managing various working conditions. The model is transient inside the components and responds with hourly weather and demand data. The main purpose of this work is providing an alternative design methodology that focuses on the collector field, and storage size b...
Preparation and characterization of cost effective spray pyrolyzed absorber layer for thin film solar cells
Sankir, Nurdan D.; Aydin, Erkan; Unver, Hulya; Uluer, Ezgi; Parlak, Mehmet (Elsevier BV, 2013-09-01)
In this study, highly (1 1 2) oriented crystalline copper indium disulfide (CuInS2) thin films with high mobility have been deposited via ultrasonic spray pyrolysis. Structural and electrical properties of CuInS2 thin films were examined to utilize them in solar cell applications. Various amounts of precursor solution ranging from 0.25 to 2.02 ml/cm(2) were used to form CuInS2 thin films onto the soda lime glass substrates. Scanning electron microscopy (SEM) analysis revealed that all sprayed films were pin...
Effects of different laser modified surface morphologies and post-texturing cleanings on c-Si solar cell performance
Radfar, Behrad; ES, FIRAT; Turan, Raşit (Elsevier BV, 2020-01-01)
Performance of crystalline Silicon solar cells depends on numerous factors. One key factor is a surface structure which can restrict electrical and optical properties due to recombination of generated carriers and reflection of incident light. Surface texturing reduces reflection from a flat surface which increases absorption of light. Anisotropic alkaline etchants are commonly used for texturing mono-crystalline Silicon wafers which produce pyramid structures on the surface. Because of randomly oriented cr...
Enhanced metal assisted etching method for high aspect ratio microstructures: Applications in silicon micropillar array solar cells
Baytemir, Gulsen; Çiftpınar, Emine Hande; Turan, Raşit (Elsevier BV, 2019-12-01)
A solar cell device, fabricated on high density array cylindrical pillars, enables photogenerated carrier collection in the radial direction, thus shortening the path length of the carriers reaching the junction. It also provides advantages over conventional planar junction solar cells, such as reduced surface reflectance and enhanced light trapping. In this study, highly ordered Si micropillars were fabricated by photolithography and metal assisted etching (MAE) methods. It is shown that the use of ethanol...
Thermoelectric polymer films with a significantly high Seebeck coefficient and thermoelectric power factor obtained through surface energy filtering
Guan, Xin; Yıldırım, Erol; Fan, Zeng; Lu, Wanheng; Li, Bichen; Zeng, Kaiyang; Yang, Shuo-Wang; Ouyang, Jianyong (Royal Society of Chemistry (RSC), 2020-07-01)
Thermoelectric (TE) polymers have unique advantages in converting heat into electricity. But their Seebeck coefficient is lower than that of inorganic TE materials by about one order of magnitude. Here, surface energy filtering is proposed to significantly enhance the Seebeck coefficient of TE polymers. The as-prepared poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS, the most popular TE polymer) films from aqueous solution have a low Seebeck coefficient of 15-18 mu V K(-1)and a power fact...
Citation Formats
A. A. Boghossian et al., “Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting,” ADVANCED ENERGY MATERIALS, pp. 881–893, 2013, Accessed: 00, 2020. [Online]. Available: