Development of non-invasive and biocompatible thin film micro-extraction contact lens-type devices compatible for in vivo metabolomics investigations from eye surface

Date

2020-7

Author

Nalbant, Atakan Arda

Metadata

Show full item record

Item Usage Stats

202
views

0
downloads

In this thesis, the aim was to develop biocompatible extractive thin films in contact lens geometries with application potential for in vivo sampling of small molecules from the eye surface with the ultimate goal of being suitable for untargeted metabolomics and biomarker discoveries. For this purpose, polydimethylsiloxane was functionalized with amine-containing groups using in situ modification approach and thin films were characterized with Fourier transform infrared spectroscopy, energy dispersive x-ray analysis, thermogravimetric analysis, and contact angle measurements. To investigate the thin films under representative conditions of eye surface contact lens geometries and in vitro agarose gel eye model were prepared in 3D printed molds To investigate the extractive capabilities of the thin films, various metabolites found on the eye surface such as representative lipids, amino acids, neurotransmitters, and other metabolites were used. Extractive capabilities of modified and unmodified polydimethylsiloxane thin films were compared by extractions from aqueous solutions and in vitro eye models after separation and detection of the analytes with liquid chromatography/mass spectrometry. Extractions showed that modified extractive lenses had between 60 to 130% increased extractive capability towards polar analytes and similar extractive capability towards non-polar analytes compared to unmodified polydimethylsiloxane extractive lenses. Composition of the desorption solvent and desorption time were optimized as ACN:MeOH:H2O (40:40:20, v/v/v/) and 30 minutes, respectively. Extractions using modified extractive lenses from the agarose in vitro eye model showed good repeatability with RSD% values ranging between 5 to 18% and good linearity in the tested range of 50.0-500.0 ng mL-1 for all analytes.

Subject Keywords

Biocompatible materials, Mass spectrometry, Metabolomics, Thin-film microextraction,

URI

https://hdl.handle.net/11511/69012

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Numerical and experimental investigation on laser damage threshold of highly reflective multilayer thin films Ocak, Mustafa; Sert, Cüneyt; Okutucu Özyurt, Hanife Tuba; Department of Mechanical Engineering (2016) The laser induced temperature distributions on the optical thin films are investigated in this study. Effects of optical design modifications on thermal performance of highly reflective (HR) multilayer thin films are analyzed. Firstly, a conventional 19 layer HR coating is selected as a reference and the laser induced temperature distribution is evaluated on it. Then, alternative HR designs are developed by employing non-quarter wave layers, over coat (OC) layers and two high index materials in the coating ...
Nano-scale phase separation and glass forming ability of iron-boron based metallic glasses Aykol, Muratahan; Akdeniz, Mahmut Vedat; Department of Metallurgical and Materials Engineering (2008) This study is pertinent to setting a connection between glass forming ability (GFA) and topology of Fe-B based metallic glasses by combining intimate investigations on spatial atomic arrangements conducted via solid computer simulations with experimentations on high GFA bulk metallic glasses. In order to construct a theoretical framework, the nano-scale phase separation encountered in metallic glasses is investigated for amorphous Fe80B20 and Fe83B17 alloys via Monte Carlo equilibration and reverse Monte Ca...
Development of high performance active materials for microbolometers Eroğlu, Numan; Akın, Tayfun; Turan, Raşit; Department of Micro and Nanotechnology (2011) This thesis reports the development of Vanadium Tungsten Oxide (VWO) film as an active detector material for uncooled infrared detectors by using the reactive DC magnetron co-sputtering method. VWO is a doped form of the Vanadium Oxide (VOx) which is known as a prominent material for uncooled infrared detectors with its high TCR, low resistivity, and low noise properties. VOx is a widely preferred material for commercialized uncooled infrared detectors along with its drawbacks. Fabrication is fairly difficu...
Investigation of linear and nonlinear optical properties of PbWO4 single crystal Delice, S.; Isik, M.; Hasanlı, Nızamı (2022-11-01) © 2022 Elsevier B.V.In this manuscript, PbWO4 single crystals having great importance for device applications were studied in terms of linear and nonlinear optical properties. For this purpose, transmission and reflection experiments were performed in the wavelength range of 350–1000 nm. X-ray diffraction analysis presented crystalline structure as scheelite type tetragonal. Spectral dependencies of absorption coefficient, skin depth, refractive index, dielectric function were reported. Optical band gap of ...
3D-graphene-laser patterned p-type silicon Schottky diode Orhan, Elif Oz; Efil, Esra; Bayram, Ozkan; Kaymak, Nuriye; Berberoğlu, Halil; Candemir, Ozun; Pavlov, Ihor; Ocak, Sema Bilge (Elsevier BV, 2021-01-01) © 2020 Elsevier LtdThe influence of the laser patterning (LP) process on the quality of graphene (Gr) film and Schottky diode characteristics was researched in this study. First of all, p-type silicon (Si) was patterned by homemade femtosecond laser source. To compare the resulting effect, non-patterned n-Si and p-Si were used as substrates. To achieve vertically oriented three-dimensional (3D) Gr nanosheets (VGNs) onto the laser patterned p-type Si, non-patterned n-Si, and p-Si substrates, we used Radio-Fr...

Citation Formats

A. A. Nalbant, “Development of non-invasive and biocompatible thin film micro-extraction contact lens-type devices compatible for in vivo metabolomics investigations from eye surface,” M.S. - Master of Science, Middle East Technical University, 2020.