Glucose Oxidase Immobilization on Thiazolothiazole Containing Multichromic Polymer Matrice for Glucose Detection

Söylemez, Saniye
Kaya, Hava
Udum, Yasemin
Toppare, Levent Kamil


Glucose isomerase production on a xylan-based medium by Bacillus thermoantarcticus
Çalık, Pınar; Haykir, Nazife Isik; BOYACI, İSMAİL HAKKI (Elsevier BV, 2009-01-15)
Effects of medium components on intracellular glucose isomerase (GI) production were investigated by Bacillus thermoantarcticus. The highest GI activity was obtained as 1630 U dm(-3) in the medium containing (g dm(-3)): 10.6, birchwood-xylan: 5.6, yeast extract: 5.9 (NH(4))(2)SO(4) at T = 55 C in 33 cm(-3) shake-flasks. When birchwood-xylan was replaced with oat spelt- or beechwood-xylan, GI activity decreased to 1372 and 1308 U dm(-3). respectively. Effects of pH at uncontrolled-pH (pH(UC) = 6.0) and contr...
Glucose determination by an immobilized enzyme electrode: immobilization of glucose oxidase in acrylamide and hema.
Arıca, Mehmet Yakup; Department of Biological Sciences (1986)
Glucose determination based on a two component self-assembled monolayer functionalized surfaceenhanced Raman spectroscopy (SERS) probe
TORUL, HİLAL; ÇİFTÇİ, HAKAN; Dudak, Fahriye Ceyda; Adiguzel, Yekbun; Külah, Haluk; BOYACI, İSMAİL HAKKI; TAMER, UĞUR (2014-01-01)
In this report, we present a new detection method for blood glucose, using gold nanorod SERS, a surface enhanced Raman scattering probe embedded in two component self-assembled monolayers (SAMs). Gold nanorod particles and a gold coated slide surface were modified with the two component SAMs consisting of 3-mercaptophenylboronic acid (3-MBA) and 1-decanethiol (1-DT). The immobilization of 3-MBA/1-DT surface-functionalized gold nanoparticles onto 3-MBA/1-DT modified gold-coated slide surfaces was achieved by...
Glucose-Responsive Trehalose Hydrogel for Insulin Stabilization and Delivery
Lee, Juneyoung; Ko, Jeong Hoon; Mansfield, Kathryn M.; Nauka, Peter C.; Bat, Erhan; Maynard, Heather D. (Wiley, 2018-05-01)
Effective delivery of therapeutic proteins is important for many biomedical applications. Yet, the stabilization of proteins during delivery and long-term storage remains a significant challenge. Herein, a trehalose-based hydrogel is reported that stabilizes insulin to elevated temperatures prior to glucose-triggered release. The hydrogel is synthesized using a polymer with trehalose side chains and a phenylboronic acid end-functionalized 8-arm poly(ethylene glycol) (PEG). The hydroxyls of the trehalose sid...
Glucose-based feeding strategy development in recombinant human growth hormone production by ichia pastoris for semi-batch bioreactor operation
Keskin, Abdullah; Çalık, Pınar; Özdamar, Tunçer; Department of Chemical Engineering (2014)
The aim of this study was to develop an effective feeding strategy to enhance recombinant human growth hormone (rhGH) production by Pichia pastoris Mut+ strain harboring pGAPZαA::hGH which is integrated to glyceraldehyde-3-phosphate dehydrogenase gene (GAP) locus of P. pastoris. In this context, eight semi-batch bioreactor experiments were conducted at pilot-scale; their batch phase was same in which glycerol was used as the sole carbon source. For semi-batch operation phase, eight feeding strategies were d...
Citation Formats
S. Söylemez, H. Kaya, Y. Udum, and L. K. Toppare, “Glucose Oxidase Immobilization on Thiazolothiazole Containing Multichromic Polymer Matrice for Glucose Detection,” 2019, Accessed: 00, 2021. [Online]. Available: