Glucose determination by an immobilized enzyme electrode: immobilization of glucose oxidase in acrylamide and hema.

Arıca, Mehmet Yakup


Glucose determination based on a two component self-assembled monolayer functionalized surfaceenhanced Raman spectroscopy (SERS) probe
TORUL, HİLAL; ÇİFTÇİ, HAKAN; Dudak, Fahriye Ceyda; Adiguzel, Yekbun; Külah, Haluk; BOYACI, İSMAİL HAKKI; TAMER, UĞUR (2014-01-01)
In this report, we present a new detection method for blood glucose, using gold nanorod SERS, a surface enhanced Raman scattering probe embedded in two component self-assembled monolayers (SAMs). Gold nanorod particles and a gold coated slide surface were modified with the two component SAMs consisting of 3-mercaptophenylboronic acid (3-MBA) and 1-decanethiol (1-DT). The immobilization of 3-MBA/1-DT surface-functionalized gold nanoparticles onto 3-MBA/1-DT modified gold-coated slide surfaces was achieved by...
Glucose Oxidase Immobilization on Thiazolothiazole Containing Multichromic Polymer Matrice for Glucose Detection
Söylemez, Saniye; Kaya, Hava; Udum, Yasemin; Toppare, Levent Kamil (2019-09-05)
Glucose isomerase production on a xylan-based medium by Bacillus thermoantarcticus
Çalık, Pınar; Haykir, Nazife Isik; BOYACI, İSMAİL HAKKI (Elsevier BV, 2009-01-15)
Effects of medium components on intracellular glucose isomerase (GI) production were investigated by Bacillus thermoantarcticus. The highest GI activity was obtained as 1630 U dm(-3) in the medium containing (g dm(-3)): 10.6, birchwood-xylan: 5.6, yeast extract: 5.9 (NH(4))(2)SO(4) at T = 55 C in 33 cm(-3) shake-flasks. When birchwood-xylan was replaced with oat spelt- or beechwood-xylan, GI activity decreased to 1372 and 1308 U dm(-3). respectively. Effects of pH at uncontrolled-pH (pH(UC) = 6.0) and contr...
Glucose-Responsive Trehalose Hydrogel for Insulin Stabilization and Delivery
Lee, Juneyoung; Ko, Jeong Hoon; Mansfield, Kathryn M.; Nauka, Peter C.; Bat, Erhan; Maynard, Heather D. (Wiley, 2018-05-01)
Effective delivery of therapeutic proteins is important for many biomedical applications. Yet, the stabilization of proteins during delivery and long-term storage remains a significant challenge. Herein, a trehalose-based hydrogel is reported that stabilizes insulin to elevated temperatures prior to glucose-triggered release. The hydrogel is synthesized using a polymer with trehalose side chains and a phenylboronic acid end-functionalized 8-arm poly(ethylene glycol) (PEG). The hydroxyls of the trehalose sid...
Glucose-based feeding strategy development in recombinant human growth hormone production by ichia pastoris for semi-batch bioreactor operation
Keskin, Abdullah; Çalık, Pınar; Özdamar, Tunçer; Department of Chemical Engineering (2014)
The aim of this study was to develop an effective feeding strategy to enhance recombinant human growth hormone (rhGH) production by Pichia pastoris Mut+ strain harboring pGAPZαA::hGH which is integrated to glyceraldehyde-3-phosphate dehydrogenase gene (GAP) locus of P. pastoris. In this context, eight semi-batch bioreactor experiments were conducted at pilot-scale; their batch phase was same in which glycerol was used as the sole carbon source. For semi-batch operation phase, eight feeding strategies were d...
Citation Formats
M. Y. Arıca, “ Glucose determination by an immobilized enzyme electrode: immobilization of glucose oxidase in acrylamide and hema.,” Middle East Technical University, 1986.