Collagen Based Multilayer Scaffolds for Meniscus Tissue Engineering: In Vivo Test Results. Biomater Med Appl 2: 1

Albana Ndreu, Halili
Karahan, Siyami
Kürüm, Barış
Hasırcı, Vasıf Nejat
Meniscus is an important component of the knee joint since it performs several crucial functions such as shock absorption, load bearing and transmission, maintenance of joint stability, and lubrication. The results of common meniscal injury repair approaches are not fully satisfactory with low mechanical properties and long regeneration times. A 3D collagen-based construct consisting of multilayers of lyophilized sponges separated by electrospun fibrous mats was prepared previously to serve as a substitute for meniscus. Mechanical properties of the construct were studied in vitro and a 3 to 4 fold increase was observed when a double crosslinking method was used. Rabbit meniscal cells were cultured in vitro, expanded and seeded onto the polymer scaffolds. 2 weeks later the substitute was implanted to the medial compartment of the rabbit knee joint. The implants were studied 3 and 10 weeks after transplantation. Histological and microscopical characterization showed a significant difference between the groups (Group I: control; Group II: cell free substitute and Group III: cell seeded substitute) with Week3 sample scores. Group III healing score was significantly lower than I and II, which was probably due to the the fibrous tissue surrounding the cell seeded material but this resulted in lower immunological responses. Moreover, the scores decreased from Week3 to Week10 indicating healing. Even though there were no statistically significant differences, the lowest values were observed with the tissue engineered substitute. Therefore, it can be concluded that in vivo studies showed the potential of the cell seeded artificial meniscus.
Biomaterials Medical Applications


Poly(L-Lactic acid) (PLLA)-based meniscus tissue engineering
Bahçecioğlu, Gökhan; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Biotechnology (2011)
Meniscus is a fibrocartilaginous tissue which plays an important role in joint stability, lubrication, and load bearing and transmission. Meniscal tears are commonly encountered in sports activities, or caused by degeneration of the cartilage with ageing. They lead to pain, loss of work, disturbed biomechanics of the knee and inability to walk or even move the legs. As the meniscal tissue is avascular in the inner portion, injury to this part does not heal by itself, and therefore treatments are needed. In ...
Collagen-based meniscus tissue engineering: design and application
Halili Ndreu, Albana; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat; Department of Biotechnology (2011)
Meniscus is a wedge shaped structure, with a convex base attached to a flat tibial surface, and with a concave femoral surface, on which femur and tibia articulate. It has several functions including joint lubrication, shock absorption, load transmission and joint stability. Various methods were tried to treat meniscal tears but each has its own drawbacks. Tissue engineering seems to be a promising solution that avoids all the problems associated with the other approaches. In this study, a three dimensional...
Vibration reduction by using two tuned mass dampers with dry friction damping
Doğan, Muhammed Emin; Ciğeroğlu, Ender (null; 2019-02-20)
Vibration reduction of a single-degree-of-freedom system connected to two tuned mass dampers (TMDs) equipped with dry friction dampers is considered in this work. The system is subjected to sinusoidal base excitation. Parameters of TMDs are optimized to minimize the peak values of the response of the primary system. Harmonic balance method (HBM) is used to obtain the steady state solution of the three-degrees-of-freedom nonlinear system in frequency domain. Newton’s method with arc length continuation is ut...
3D plotted PCL scaffolds for stem cell based bone tissue engineering
Yilgor, Pinar; Sousa, Rui A.; Reis, Rui L.; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2007-10-04)
The ability to control the architecture and strength of a bone tissue engineering scaffold is critical to achieve a harmony between the scaffold and the host tissue. Rapid prototyping (RP) technique is applied to tissue engineering to satisfy this need and to create a scaffold directly from the scanned and digitized image of the defect site. Design and construction of complex structures with different shapes and sizes, at micro and macro scale, with fully interconnected pore structure and appropriate mechan...
Biological Responses of Ceramic Bone Spacers Produced by Green Processing of Additively Manufactured Thin Meshes
Minguella-Canela, Joaquim; Calero, Jose Antonio; Korkusuz, Feza; Korkusuz, Petek; Kankılıç, Berna; Bilgiç, Elif; De los Santos-López, M. Antonia (MDPI AG, 2020-5-30)
Bone spacers are exclusively used for replacing the tissue after trauma and/or diseases. Ceramic materials bring positive opportunities to enhance greater osteointegration and performance of implants, yet processing of porous geometries can be challenging. Additive Manufacturing (AM) opens opportunities to grade porosity levels in a part; however, its productivity may be low due to its batch processing approach. The paper studies the biological responses yielded by hydroxyapatite with beta -TCP (tricalcium ...
Citation Formats
H. Albana Ndreu, S. Karahan, B. Kürüm, and V. N. Hasırcı, “Collagen Based Multilayer Scaffolds for Meniscus Tissue Engineering: In Vivo Test Results. Biomater Med Appl 2: 1,” Biomaterials Medical Applications, pp. 1–9, 2018, Accessed: 00, 2021. [Online]. Available: