Collagen-based meniscus tissue engineering: design and application

Halili Ndreu, Albana
Meniscus is a wedge shaped structure, with a convex base attached to a flat tibial surface, and with a concave femoral surface, on which femur and tibia articulate. It has several functions including joint lubrication, shock absorption, load transmission and joint stability. Various methods were tried to treat meniscal tears but each has its own drawbacks. Tissue engineering seems to be a promising solution that avoids all the problems associated with the other approaches. In this study, a three dimensional (3D) collagen-based structure was prepared by tissue engineering to mimic the natural human meniscus. Three different foams prepared under different conditions were combined and nano/microfibrous layers were placed in between them. To mimic the properties of the natural tissue, the top layer was composed of collagen-chondroitin sulfate-hyaluronic acid (Coll-CS-HA) prepared by freezing at -20ºC followed by lyophilization. The middle and bottom layers were made with just collagen after freezing at -20ºC and -80ºC, respectively and lyophilization. Aligned nano/microfibers were prepared using collagen-poly(L-lactic-co-glycolic acid (Coll-PLGA). Various crosslinking procedures such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), genipin (GP), glutaraldehyde (GLU) either alone or in combination with dehydrothermal treatment (DHT) were used and based on both compressive and tensile properties, the best crosslinker was chosen to be DHT+EDC/NHS. Mechanical properties (compressive, tensile and shear) of the dry foams and the final 3D construct were evaluated. The highest mechanical properties were obtained with the 3D construct. Then all these foams and the 3D construct were seeded with human fibrochondrocytes to study the cell behavior such as attachment, proliferation, and extracellular matrix (ECM) and glucosaminoglycan (GAG) production. Furthermore, the influence of cell seeding on the compressive properties of wet individual foams and the 3D construct was observed. As expected, the highest cell proliferation and compressive properties were obtained with the 3D construct. Finally, the 3D constructs, seeded with fibrochondrocytes, were implanted in New Zealand rabbits after meniscectomy. The initial microscopical examination show that the 3D construct has a significant potential as a meniscus substitute.


Poly(L-Lactic acid) (PLLA)-based meniscus tissue engineering
Bahçecioğlu, Gökhan; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Biotechnology (2011)
Meniscus is a fibrocartilaginous tissue which plays an important role in joint stability, lubrication, and load bearing and transmission. Meniscal tears are commonly encountered in sports activities, or caused by degeneration of the cartilage with ageing. They lead to pain, loss of work, disturbed biomechanics of the knee and inability to walk or even move the legs. As the meniscal tissue is avascular in the inner portion, injury to this part does not heal by itself, and therefore treatments are needed. In ...
Collagen Based Multilayer Scaffolds for Meniscus Tissue Engineering: In Vivo Test Results. Biomater Med Appl 2: 1
Albana Ndreu, Halili; Karahan, Siyami; Kürüm, Barış; Hasırcı, Vasıf Nejat (2018-04-01)
Meniscus is an important component of the knee joint since it performs several crucial functions such as shock absorption, load bearing and transmission, maintenance of joint stability, and lubrication. The results of common meniscal injury repair approaches are not fully satisfactory with low mechanical properties and long regeneration times. A 3D collagen-based construct consisting of multilayers of lyophilized sponges separated by electrospun fibrous mats was prepared previously to serve as a substitute ...
Inter-granular cracking through strain gradient crystal plasticity and cohesive zone modeling approaches
Yalçınkaya, Tuncay; Fırat, Arzu (Elsevier BV, 2019-10-01)
Even though intergranular fracture is generally regarded as a macroscopically brittle mechanism, there are various cases where the fracture occurs at the grain boundaries with considerable plastic deformation at the macroscopic scale. There exists several microstructural reasons for grain boundaries to host crack initiation. They can interact with impurities and defects, can provide preferential location for precipitation, can behave as a source of dislocations and can impede the movement of dislocations as...
Contact guidance enhances the quality of a tissue engineered corneal stroma
Vrana, E.; Builles, N.; Hindie, M.; Damour, O.; AYDINLI, ATİLLA; Hasırcı, Vasıf Nejat (2008-02-01)
Corneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. This highly complex nature of cornea is known to be important for its transparency and mechanical integrity. Thus, an artificial cornea design has to take into account this complex structure. In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. Keratocytes proliferated well on films and reached confluency after 7 days in the incubation medium....
Nonlinear time-varying dynamic analysis of a spiral bevel geared system
Yavuz, Siar Deniz; Sarıbay, Zihni Burcay; Ciğeroğlu, Ender (Springer Science and Business Media LLC, 2018-06-01)
In this paper, a nonlinear time-varying dynamic model of a drivetrain composed of a spiral bevel gear pair, shafts and bearings is developed. Gear shafts are modeled by utilizing Timoshenko beam finite elements, and the mesh model of a spiral bevel gear pair is used to couple them. The dynamic model includes the flexibilities of shaft bearings as well. Gear backlash and time variation of mesh stiffness are incorporated into the dynamic model. Clearance nonlinearity of bearings is assumed to be negligible, w...
Citation Formats
A. Halili Ndreu, “ Collagen-based meniscus tissue engineering: design and application,” Ph.D. - Doctoral Program, Middle East Technical University, 2011.