Biological Responses of Ceramic Bone Spacers Produced by Green Processing of Additively Manufactured Thin Meshes

Download

10.3390:ma13112497.pdf

Date

2020-5-30

Author

Minguella-Canela, Joaquim
Calero, Jose Antonio
Korkusuz, Feza
Korkusuz, Petek
Kankılıç, Berna
Bilgiç, Elif
De los Santos-López, M. Antonia

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

182
views

127
downloads

Bone spacers are exclusively used for replacing the tissue after trauma and/or diseases. Ceramic materials bring positive opportunities to enhance greater osteointegration and performance of implants, yet processing of porous geometries can be challenging. Additive Manufacturing (AM) opens opportunities to grade porosity levels in a part; however, its productivity may be low due to its batch processing approach. The paper studies the biological responses yielded by hydroxyapatite with beta -TCP (tricalcium phosphate) ceramic porous bone spacers manufactured by robocasting 2-layer meshes that are rolled in green and sintered. The implants are assessed in vitro and in vivo for their compatibility. Human bone marrow mesenchymal stem cells attached, proliferated and differentiated on the bone spacers produced. Cells on the spacers presented alkaline phosphatase staining, confirming osteogenic differentiation. They also expressed bone-specific COL1A1, BGAP, BSP, and SPP1 genes. The fold change of these genes ranged between 8 to 16 folds compared to controls. When implanted into the subcutaneous tissue of rabbits, they triggered collagen fibre formation and mild fibroblastic proliferation. In conclusion, rolled AM-meshes bone spacers stimulated bone formation in vitro and were biocompatible in vivo. This technology may give the advantage to custom produce spacers at high production rates if industrially upscaled.

Subject Keywords

General Materials Science, Additive manufacturing, Implants, Bioceramics, Porous scaffolds, Bone tissue engineering, Cell proliferation, Biological responses

URI

https://hdl.handle.net/11511/51451

Journal

Materials

DOI

https://doi.org/10.3390/ma13112497

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Mesenchymal Stem Cells and Nano-Bioceramics for Bone Regeneration Kankilic, Berna; Kose, Sevil; KORKUSUZ, PETEK; Timucin, Muharrem; Korkusuz, Feza (2016-01-01) Orthopedic disorders and trauma usually result in bone loss. Bone grafts are widely used to replace this tissue. Bone grafts excluding autografts unfortunately have disadvantages like evoking immune response, contamination and rejection. Autografts are of limited sources and optimum biomaterials that can replace bone have been searched for several decades. Bioceramics, which have the similar inorganic structure of natural bone, are widely used to regenerate bone or coat metallic implants. As people continuo...
Design of a modular orthopedic implant Erkan, Onur Mert; Tönük, Ergin; Department of Mechanical Engineering (2015) Bone fracture due to trauma and bone defects by birth are very common in orthopedics, making their treatment crucial. In this study, a novel design to treat upper arm fractures is introduced and assessed mechanically. The design offers medical doctors longitudinal and angular flexibility when compared to widely used orthopedic plates. Hence the new design covers a variety of fracture types. Earlier conceptual designs are reviewed to demonstrate the progress of design. Mechanical performance of the final des...
Biodegradable hydroxyapatite - Polymer composites Durucan, Caner (2001-04-01) The fracture of bone due to trauma or due to natural aging is one of the most frequent types of tissue failures. Treatment frequently requires the implantation of ct temporary or permanent prosthesis. The implanted materials may include the components of artificial joints, plates, and screws for fracture fixation. Typically, such implants are intended only to provide structural support or to serve as templates for bone re-growth. In general they are intended to remain in place for the life of the patient or...
Additive manufacturing of biodegradable magnesium implants and scaffolds: Review of the recent advances and research trends Sezer, Nurettin; Evis, Zafer; Koc, Muammer (2021-03-01) Synthetic grafting needs improvements to eliminate secondary surgeries for the removal of implants after healing of the defected tissues. Tissue scaffolds are engineered to serve as temporary templates, which support the affected tissue and gradually degrade through the healing period. Beside mechanical function to withstand the anatomic loading conditions, scaffolds should also provide a decent biological function for the diffusion of nutrients and oxygen to the cells, and excretion of the wastes from the ...
Improvement of bioactivity with dual bioceramic incorporation to nanofibrous PCL scaffolds Altunordu, Gercem; Tezcaner, Ayşen; Evis, Zafer; Keskin, Dilek (2023-03-01) Bone tissue injuries, diseases or related clinical interventions require bone tissue engineering (BTE) approaches for regeneration of large bone defects, especially for compromised situations. Most BTE applications in literature focused on composites of polymers with a single type of bioceramic. However, native bone matrix has various inorganic components. Accordingly, this study aimed to investigate the use of dual bioceramics in BTE scaffolds prepared by wet-electrospinning of Poly-caprolactone (PCL) and ...

Citation Formats

J. Minguella-Canela et al., “Biological Responses of Ceramic Bone Spacers Produced by Green Processing of Additively Manufactured Thin Meshes,” Materials, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51451.