Identification of R Plasmids of Pseudomonas aeruginosa isolates from clinical sources by recombinant DNA techniques

Date

1994-01-01

Author

İzgü, Kadri Fatih

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The R-plasmids, which transfer resistance to p. aeruginosa strains agaiiıst generally used antibiotics in the treatmet of the infections caıısed by this gram (-) bacteria were investigated. The P. aeruginosa samples used in this study were isolat-ed from the clinical cases of Gülhane Military Medical Academy (GATA), such as throat, ear and urinary tract infections. Those clinical specimens of P. aeruginosa were tested far their sensitivities to fourteen different antibiotics that are used against P. aeurigonosa infec-tions in hospitals. It was found that ampicillin was the only antibiotic that all of the P. aerugi,nosa samples were resistant to. Using ampicillin as a selectable agent, plasmid isolations were done from all of the P. aeruginosa isolates. The isolated plasmids were then digested by the restric-tion endonuclease Bam HI. The agarose gel electropho-retic analysis showed that ali of the plasmids had only two and the same Bam HI sites. By the fragment size analysis of the Bam HI cut plas-mids, it was det~rmined that all of the P. aeruginosa iso-lates, that were resistant to ampicillin, contained identi-cal plasmids of 6 .25 kb. This result indicated that the P. aeruginosa samples used in this study carry an R-plasmid of 6.25 kb, which is not similar to those generally foun_d R-plasmids in this species.

Subject Keywords

P. aeruginosa, Antibiotic resis-tance, R-plasmid

URI

https://hdl.handle.net/11511/83391
http://dergi.fabad.org.tr/pdf/volum19/Issue1/4.pdf

Journal

FABAD J

Collections

Department of Biology, Article

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Citation Formats

K. F. İzgü, “Identification of R Plasmids of Pseudomonas aeruginosa isolates from clinical sources by recombinant DNA techniques,” FABAD J, pp. 15–22, 1994, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/83391.