Effects of hospital wastewaters on the disseminatıon of tetracycline resistance genes

Date

2018-04-27

Author

Kayalı, Osman
İçgen, Bülent

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Today emergence and dissemination of antibiotic resistance are considered one of the major threat to the public health. Antibiotics are also used in agriculture, animal husbandry and veterinary practices. This kind of intensive usage increases the exposure of antibiotic resistant bacteria and resistance genes to the natural habitats. Hospital wastewaters act as reservoirs for antibiotic resistance genes and resistant bacteria due to its infectious and toxic content. Tetracyclines are discovered in 1940s and the first resistant bacteria is isolated in early 1950s. They have widespread use against Gram-positive and Gramnegative bacteria. The drug tetracycline inhibits bacterial protein synthesis by binding in ribosomal 30s subunit. There are 3 well studied resistance mechanisms for tetracyclines as efflux proteins, ribosomal protection and enzymatic inactivation. Most of the genes that provide tetracycline resistance are associated with mobile elements. This underlines horizontal gene transfer as the main mechanism responsible for the wide distribution of tetracycline resistance genes. The gene tetA is one of the tetracycline resistance gene that encodes for an efflux protein. In this study, therefore, the primers targeting tetA gene were used for quantitative analysis of seasonally collected effluents of hospital wastewaters. Outcome of the study will be used to determine the effects of hospital wastewaters on the tetracycline resistance dissemination in the environment.

Subject Keywords

Hospital Wastewaters, Antibiotic Resistance, Tetracycline Resistance Genes, Teta

URI

https://hdl.handle.net/11511/84868
https://www.eurasianbiochem.org/bildiri%20taslaklar%C4%B1/Abstract_Book_EurasianBioChem_2018.pdf

Conference Name

International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2018) (26 - 27 Nisan 2018)

Collections

Department of Environmental Engineering, Conference / Seminar

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Citation Formats

O. Kayalı and B. İçgen, “Effects of hospital wastewaters on the disseminatıon of tetracycline resistance genes,” presented at the International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2018) (26 - 27 Nisan 2018), Ankara, Türkiye, 2018, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/84868.