In vitro regeneration of Anatolian black pine [Pinus nigra Arnold subsp. pallasiana (Lamb.) Holmboe] from excised embryos

Adventitious buds and shoots were regenerated in vitro from the excised embryos of Anatolian black pine (Pinus niqra subsp. pallasiana) on Schenk and Hildebrand (SH), Gresshoff and Doy (GD), White, Gamborg (G), White (W) and Woody Plant Medium (WPM) containing 22.4-44.4 μM benzyladenin (BA) and 0.054 μM α-naphthalene acetic acid (NAA). BA concentrations ranging from 8.9 μM to 44.4 μM were adequate for growth and development of adventitious shoots. For root development, 2.7 μM NAA was the best. Among the tested culture media, White and WPM were the most suitable media for both in vitro adventitious shoot and root formation from excised tissues of Anatolian black pine embryos.
Turkish Journal of Botany


The pattern of genetic variation in Pinus nigra subspecies pallasiana natural populations from the Kazdagi and Bolkar mountains, Turkey: Implications for in situ gene conservation
Kaya, Zeki; Cengel, BN; Velioglu, E; Tolun, GG (1999-01-01)
Eleven populations of black pine (Pinus nigra Arnold subspecies pallasiana) were studied at 17 polymorphic isoenzyme gene loci. The mean number of alleles per locus (A) was 1.6. Observed heterozygosity (H-obs) was the lowest in the Gurgendag population from Kazdagi (0.119) and the highest in the Qamhyayla population from Bolkar Mountains (0.287). The expected heterozygosities (H-exp) ranged from 0.296 (Asar, from Kazdagi) to 0.181 (Cehennemdere, from Bolkar Mountains). F-ST was 0.0592 for Kazdagi and 0.0358...
Genetic structure of black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled from the Bolkar Mountains
Tolun, AA; Velioğlu, E; Çengel, Burcu N; Kaya, Zeki (2000-01-01)
In order to determine the magnitude and pattern of genetic diversity among Anatolian Black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled in Bolkar Mountains and to recommend the potential populations which may be suitable for in situ conservation of genetic resources in this species, isoenzymes from 14 enzyme systems were investigated by starch gel electrophoresis. For this reason, open pollinated seed megagametophytes of half-sib families originated from the four populations (Camliyay...
Pattern and magnitude of genetic diversity in Pinus nigra ARNOLD subspecies pallasiana populations from Kazdagi: Implications for in situ conservation
Cengel, Burcu; Velioğlu, Ercan; Tolun, Ayper A; Kaya, Zeki (2000-01-01)
To determine the genetic structure of black pine (Pinus nigra ARNOLD subspecies pallasiana), populations sampled from Kazdag (Eybekli, Asar, Katrandag, Kalklm, Gurgendag, Kapidag, Mihlidere). Isozymes from 16 enzyme systems were investigated from haploid female megagametophytes by starch gel electrophoresis.
Genetic variation in wood specific gravity of half-sib families of Pinus nigra subsp pallasiana tested at the juvenile stage: Implications for early selection
Kaya, Zeki; Temerit, A; Vurdu, H (2003-01-01)
Seeds from 7 populations (total of 281 half-sib families, progeny test) and 35 seed stands (provenance test) representing natural range of Anatolian black pine (Pinus nigra subsp. pallasiana) were sown in a forest nursery in Ankara in 1990 and raised until age 3. Stem wood specific gravity (WSG) of all seedlings was determined at age of 3. The results of this study indicated that WSG did not vary significantly neither among the 7 populations (ranging from 0.41 to 0.42) nor among 35 seed stands (ranging from...
Determination of esterase enzyme polymorphism in house fly (Musca domestica L.) populations in Turkey
TAŞKIN, VATAN; Taskin, Belgin Gocmen; KÜÇÜKAKYÜZ, KÖKSAL; Kence, Meral (2011-01-01)
The objective of this study was to determine esterase enzyme polymorphism in 16 natural Musca domestica L. populations from the Aegean and Mediterranean regions of Turkey by using the native polyacrylamide gel electrophoresis (PAGE) technique. The high level of genetic variation among populations and species make esterase enzymes an important tool for analyzing genetic differentiation and evolutionary relationships in insects. In the present study, 22 a-esterase bands were detected by using a-naphthylacetat...
Citation Formats
Z. Kaya, “In vitro regeneration of Anatolian black pine [Pinus nigra Arnold subsp. pallasiana (Lamb.) Holmboe] from excised embryos,” Turkish Journal of Botany, pp. 197–202, 1997, Accessed: 00, 2021. [Online]. Available: