Vitamin E Derivative with Modified Side Chain Induced Apoptosis by Modulating the Cellular Lipids and Membrane Dynamics in MCF7 Cells

Gok, Seher
Kuzmenko, Oleksandr
Babinskyi, Andrii
Severcan, Feride
The vitamin E derivative with side chain modification (TC6OAc) has been shown to possess anticancer activity in our earlier in vivo studies. It was hypothesized that, as Vitamin E (VE) and VE derivative are fat soluble lipophilic molecules, they exert their function by modulating the lipid metabolism and related pathways. This study aimed to evaluate the cellular impact of this VE derivative (2,5,7,8-Tetramethyl-2-(4 '-Methyl-3 '-Pentenyl)-6-Acetoxy Chromane-TC6OH), using alpha-tocopherol as a reference compound throughout the experiments. Their effects on the cellular metabolism, the biophysical properties of cellular lipids and the functional characteristics of cells were monitored in human estrogen receptor (ER) positive breast cancer cells. It has been documented that TC6OH treatment induces tumor cell apoptosis by dissipating the mitochondrial membrane potential, modulating the lipid, transportation and degradation as well as downregulating certain anti-apoptotic and growth factor related proteins. Due to resistance of ER positive cells to the established therapies, the findings of this study are of translational value.


Vitamin E Decreases the Order of the Phospholipid Model Membranes in the Gel Phase: An FTIR Study
Severcan, Feride (Portland Press Ltd., 1997-4-1)
The effect of α-tocopherol on the frequency of the CH2 stretching bands of infrared spectra of dipalmitoylphosphotidylcholine multibilayers has been investigated, both in H2O and 2H2O buffer, to determine the reason for the discrepancy in the literature between the results of different spectroscopic techniques relating to the effect of α-tocopherol on membrane order in the gel phase. In contrast to previous FTIR studies, the present FTIR results indicate that αT increases the frequencies of the CH2 stretchi...
Embryonic stem cell-derived motoneurons provide a highly sensitive cell culture model for botulinum neurotoxin studies, with implications for high-throughput drug discovery.
Kiriş, Erkan; Burnett, JC; Kota, KP; Koh, DC; Wanner, LM; Torres-Melendez, E; Gussio, R; Tessarollo, L; Bavari, S (Elsevier BV, 2011-05-01)
Botulinum neurotoxins (BoNTs) inhibit cholinergic synaptic transmission by specifically cleaving proteins that are crucial for neurotransmitter exocytosis. Due to the lethality of these toxins, there are elevated concerns regarding their possible use as bioterrorism agents. Moreover, their widespread use for cosmetic purposes, and as medical treatments, has increased the potential risk of accidental overdosing and environmental exposure. Hence, there is an urgent need to develop novel modalities to counter ...
The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum
Kocabıyık, Semra; Russel, RJM; Danson, MJ; Hough, DW (Elsevier BV, 1996-07-05)
In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels and have investigated the effects of this mutation on the biochemical properties and thermal stability of the enzyme. Both wild-type and the mutant enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The mutant Thermoplasma citrate synthase is very similar to wild-type citrate s...
Dietary selenium- and vitamin E-induced alterations in some rabbit tissues
Turan, B; Zaloglu, N; Koc, E; Saran, Y; Akkas, N (Springer Science and Business Media LLC, 1997-09-01)
The present study was designed to investigate and compare the effects of dietary selenium (Se) and vitamin E on some physiological parameters and histological changes in liver, heart, and skin tissues, as well as the blood parameters and the related enzymes. Both sex young rabbits were fed with deficient (9.8 mu g/kg diet), adequate (225 mu g/kg diet), and rich (4.2 mg/kg diet) Se and vitamin E diets for 12-15 wk for this purpose As the plasma Se levels and the erythrocyte glutathione (GSH) peroxidase activ...
Inositol phospholipids activate plasma membrane ATPase in plants
Memon, Abdul Razaque; Chen, Qiuyun; Boss, Wendy F. (Elsevier BV, 1989-8)
Phosphatidylinositol-4-monophosphate and phosphatidylinositol-4,5-bisphosphate increased the activity of the vanadate-sensitive ATPase associated with plasma membranes isolated from both sunflower hypocotyls and carrot suspension culture cells. The response was not due to the metabolism of the polyphosphoinositides since diacylglycerol, inositol-1,4-bisphosphate, inositol-1,4,5-trisphosphate, glycerophosphoinositol monophosphate and glycerophosphoinositol bisphosphate had no effect. These data suggest that ...
Citation Formats
S. Gok, O. Kuzmenko, A. Babinskyi, and F. Severcan, “Vitamin E Derivative with Modified Side Chain Induced Apoptosis by Modulating the Cellular Lipids and Membrane Dynamics in MCF7 Cells,” CELL BIOCHEMISTRY AND BIOPHYSICS, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: