Fullerol based enzyme mimics and synthesis effort of tetra aryl pyranone

Download
2021-2-12
Soydan, Medine
Although, natural enzymes have high catalytic activity and specificity, they are fragile and costly due to long purification processes. Therefore, scientists started to design and synthesize structures with enzyme-like activity called nanozymes to overcome the limitations of the natural enzymes. In the first part of this project, amino acids of catalytic site of three enzymes were conjugated into fullerol.The names of three enzymes are lactate dehydrogenase, protease and nitrilase.Then, the catalytic activity of fullerol amino acid derivatives was tested with the model reactions.Luminescence was observed and attracted attention throughout the history. Accordingly, understanding of the luminescence made great progress. Aggregation caused quenching and the aggregation induced emission processes are the relatively new concept in the comprehension of luminescence. Moreover, newmolecules which show ACQ or AIE property are designed and synthesized for various applications like imaging. In the second part of this project, it was aimed to synthesize 2,3,4,5-tetraphenyl-4H-pyran-4-one by a new synthesis route. Moreover, it was planned to investigate whether it has aggregation caused quenching or the aggregation induced emission property with fluorescence spectroscopy.

Suggestions

Enantioselective synthesis of new chiral 2-aziridinyl phosphonates and studies of their biological activities
Doğan, Özdemir; Beksultanova, Nurzhan; ALTANLAR, NURTEN; Simsek, Duygu; KARABIYIK, HASAN (2017-02-15)
A new series of chiral aziridinyl phosphonates has been synthesized and evaluated for antibacterial and antifungal activities. For the synthesis, a Gabriel-Cromwell reaction was used to form aziridinyl phosphonates in 52-83% yield. In order to evaluate antibacterial and antifungal activities, MIC values were measured. Although most of the compounds showed insignificant activity, two of them provided low to moderate antifungal activity.
Development of amperometric cholinesterase biosensors based on a conducting polymer for detection of anti-dementia drugs and pesticides
Turan, Janset; Toppare, Levent Kamil; Özkan, Necati; Department of Polymer Science and Technology (2016)
Cholinesterase enzymes are considered as one of the most important enzymes in living organisms and they are targeted by many inhibitors. Cholinesterase inhibitors are potentially toxic however they also have certain applications as treatment of dementia and Alzheimer’s diseases. These inhibitors can bind the active side of the cholinesterase enzymes forming stable complexes. This inhibitory effect can be used to construct fast and sensitive detection system. Also, conducting polymers are known to be well su...
Silver-Sodium Ion Exchange Dynamics in LTA Zeolite Membranes
Salmas, Ramin Ekhteiari; Demir, Baris; Yıldırım, Erol; Sirkecioğlu, Ahmet; Yurtsever, Mine; Ahunbay, Mehmet Göktuğ (2013-01-31)
Because of their high ion exchange capacity, silver exchanged LTA (Ag-LTA) zeolites can be incorporated in polymeric materials as antibacterial agents for various purposes such as preventing biofouling in water treatment membranes due to the formation of microorganisms like bacteria, algae. Ag-LTA can be prepared by exchanging extra-framework Na+ ions in an aqueous solution containing Ag+ ions. Since the release amount and rate of Ag+ ions from the zeolite are crucial factors determining the antibacterial e...
Poly-beta-hydroxybutyrate production by lactic acid bacteria
Aslim, B; Caliskan, F; Beyatli, Y; Gündüz, Ufuk (1998-02-15)
Poly-beta-hydroxybutyrate was determined in lactic acid bacteria belonging to the genera Lactobacillus, Lactococcus, Pediococcus and Streptococcus. Lactobacilli were grown in MRS broth, the others were grown in Elliker broth medium. Cell biomass was obtained by centrifugation. The cell walls were lysed with sodium hypochlorite. Poly-beta-hydroxybutyrate was extracted using chloroform in a Soxhlet system. Then it was converted to crotonic acid using sulfuric acid and the amount of crotonic acid was measured ...
Poly-beta-hydroxybutyrate accumulation and releasing by hydrogen producing bacteria, Rhodobacter sphaeroides OU001. A transmission electron microscopic study
Cetin, Demet; Guenduez, Ufuk; Eroğlu, İnci; Yuecel, Meral; Tuerker, Lemi (2006-11-16)
Photosynthetic bacterium Rhodobacter sphaeroides O.U.001 that is used for photobiohydrogen production can also accumulate poly-beta-hydroxybutyrate (PHB) as a by-product when cultivated anaerobically with minimal medium containing L-malic acid, sodium glutamate and some vitamins under illumination. Transmission electron microscopy studies revealed that PHB granules are made of two distinct components: a homogenous and electron lucent core is covered with a more electron dense coat. PHB granules were observe...
Citation Formats
M. Soydan, “Fullerol based enzyme mimics and synthesis effort of tetra aryl pyranone,” M.S. - Master of Science, Middle East Technical University, 2021.