Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis

Download

1-s2.0-S1873506121002166-main.pdf

Date

2021-05-01

Author

Koc, Dilara
Begentaş, Onur Can
Yurtogullari, Sukran
Temel, Musa
Akcali, Kamil Can
Demirkaya, Seref
Kiriş, Erkan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

80
views

14
downloads

Multiple sclerosis is a chronic disease characterized by inflammation, demyelination, and axonal damage in the central nervous system. Here, we established an induced pluripotent stem cell (iPSC) line METUi001-A from the peripheral blood mononuclear cells of a 25-year-old male individual with clinically diagnosed Relapsing-Remitting Multiple Sclerosis (RRMS) using the integration-free Sendai reprogramming method. We demonstrated that the iPSCs are free of exogenous Sendai reprogramming vectors, have a normal male karyotype, express pluripotency markers, and differentiate into the three germ layers. The iPSC line can serve as a valuable resource to generate cellular model systems to investigate molecular mechanisms underlying RRMS.

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107157848&origin=inward
https://hdl.handle.net/11511/91092

Journal

Stem Cell Research

DOI

https://doi.org/10.1016/j.scr.2021.102370

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Generation and Characterization of Induced Pluripotent Stem Cell Lines From Multiple Sclerosis Patients and Healthy Individuals Begentaş, Onur Can; Kiriş, Erkan; Department of Molecular Biology and Genetics (2021-9-8) Multiple Sclerosis (MS) is an autoimmune disease characterized by inflammation, demyelination, and axonal damage resulting in neurodegeneration in the central nervous system (CNS). Relapsing-remitting MS (RRMS) is the most common form of the disease known to affect more than 2 million individuals globally, and the prevalence of the disease is increasing worldwide. As a chronic condition without a cure, RRMS manifests in a relapsing-remitting form with sporadic attacks suddenly appearing, causing neurologica...
Investigation of Glutathione S-Transferase (GST) expression and activity in mouse with Multiple Sclerosis (MS) Arçak, Deniz; Adalı, Orhan; Evin, Emre; Department of Biology (2022-5-11) Multiple Sclerosis (MS) of unknown etiopathogenesis is a chronic demyelinating disease of the central nervous system. It mainly destroys myelin in the brain and spinal cord. Non-traumatic injuries have been observed in this disease for young adults. Various factors affect MS, but oxidative stress is one of the most important causes of demyelination. Glutathione S- Transferases (GSTs) can be described as a versatile enzyme family of eukaryotic and prokaryotic phase II metabolic isoenzymes. They have enzymati...
INVESTIGATION OF EFFECTS OF VITAMIN D AND VITAMIN D METABOLIZING CYP450 ISOZYMES ON MULTIPLE SCLEROSIS PATHOPHYSIOLOGY IN ANIMAL MODEL BY MOLECULAR APPROACHES Evin, Emre; Adalı, Orhan; Department of Biology (2021-8) Multiple sclerosis (MS) is a complicated, recurrent, and often progressive inflammatory demyelinating autoimmune disease of the central nervous system, yet etiopathogenesis remains unsolved. MS frequency increases with increasing latitude, leading to a hypothesis that MS is inversely correlated with the duration and intensity of sunlight and vitamin D concentrations. In this study, the relationships between vitamin D supplementation, MS, VDR, and vitamin D metabolizing CYP enzymes, including CYP2R1, CYP27A1...
Detection and classification of qrs complexes from the ecg recordings Koç, Bengi; Serinağaoğlu Doğrusöz, Yeşim; Department of Electrical and Electronics Engineering (2008) Electrocardiography (ECG) is the most important noninvasive tool used for diagnosing heart diseases. An ECG interpretation program can help the physician state the diagnosis correctly and take the corrective action. Detection of the QRS complexes from the ECG signal is usually the first step for an interpretation tool. The main goal in this thesis was to develop robust and high performance QRS detection algorithms, and using the results of the QRS detection step, to classify these beats according to their d...
Relapsing-Remitting Multiple Sclerosis diagnosis from cerebrospinal fluids via Fourier transform infrared spectroscopy coupled with multivariate analysis Yonar, Dilek; Ocek, Levent; Tiftikcioglu, Bedile Irem; Zorlu, Yasar; Severcan, Feride (2018-01-18) Multiple sclerosis (MS) is a chronic, progressive, inflammatory and degenerative disease of central nervous system. Here, we aimed to develop a method for differential diagnosis of Relapsing-Remitting MS (RRMS) and clinically isolated syndrome (CIS) patients, as well as to identify CIS patients who will progress to RRMS, from cerebrospinal fluid (CSF) by infrared (IR) spectroscopy and multivariate analysis. Spectral analyses demonstrated significant differences in the molecular contents, especially in the l...

Citation Formats

D. Koc et al., “Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis,” Stem Cell Research, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107157848&origin=inward.