Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis

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2021-05-01

Author

Koc, Dilara
Begentaş, Onur Can
Yurtogullari, Sukran
Temel, Musa
Akcali, Kamil Can
Demirkaya, Seref
Kiriş, Erkan

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Multiple sclerosis is a chronic disease characterized by inflammation, demyelination, and axonal damage in the central nervous system. Here, we established an induced pluripotent stem cell (iPSC) line METUi001-A from the peripheral blood mononuclear cells of a 25-year-old male individual with clinically diagnosed Relapsing-Remitting Multiple Sclerosis (RRMS) using the integration-free Sendai reprogramming method. We demonstrated that the iPSCs are free of exogenous Sendai reprogramming vectors, have a normal male karyotype, express pluripotency markers, and differentiate into the three germ layers. The iPSC line can serve as a valuable resource to generate cellular model systems to investigate molecular mechanisms underlying RRMS.

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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107157848&origin=inward
https://hdl.handle.net/11511/91092

Journal

Stem Cell Research

DOI

https://doi.org/10.1016/j.scr.2021.102370

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Department of Biology, Article

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Citation Formats

D. Koc et al., “Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis,” Stem Cell Research, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107157848&origin=inward.