A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting

Download

ındex.pdf

Date

2021-12-01

Author

Demircan Yalçın, Yağmur
Töral, Taylan Berkin
Sukas, Sertan
Yıldırım, Ender
Zorlu, Özge
Gündüz, Ufuk
Külah, Haluk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

306
views

49
downloads

We report the development of a lab-on-a-chip system, that facilitates coupled dielectrophoretic detection (DEP-D) and impedimetric counting (IM-C), for investigating drug resistance in K562 and CCRF-CEM leukemia cells without (immuno) labeling. Two IM-C units were placed upstream and downstream of the DEP-D unit for enumeration, respectively, before and after the cells were treated in DEP-D unit, where the difference in cell count gave the total number of trapped cells based on their DEP characteristics. Conductivity of the running buffer was matched the conductivity of cytoplasm of wild type K562 and CCRF-CEM cells. Results showed that DEP responses of drug resistant and wild type K562 cells were statistically discriminative (at p = 0.05 level) at 200 mS/m buffer conductivity and at 8.6 MHz working frequency of DEP-D unit. For CCRF-CEM cells, conductivity and frequency values were 160 mS/m and 6.2 MHz, respectively. Our approach enabled discrimination of resistant cells in a group by setting up a threshold provided by the conductivity of running buffer. Subsequent selection of drug resistant cells can be applied to investigate variations in gene expressions and occurrence of mutations related to drug resistance.

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85108812547&origin=inward
https://hdl.handle.net/11511/91248

Journal

Scientific Reports

DOI

https://doi.org/10.1038/s41598-021-92647-5

Collections

Department of Mechanical Engineering, Article

Suggestions

OpenMETU
Core

A DEP-Based Lab-On-A-Chip System For The Detection Of Multidrug Resistance In K562 Leukemia Cells Yalçın, Yağmur Demircan; Özkayar, Gürkan; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk (2016-06-09) This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100% selectivity at a flow rate of...
A Droplet based Multi-Drug Screening System Controlled with Electrostatic Microvalves Yıldırım, Ender; Külah, Haluk (2012-10-28) This paper presents a droplet-based drug effect analysis system utilizing electrostatically-actuated normallyclosed microvalves to screen the effect of multiple drugs on a single type of cell. Proposed system minimizes the need for off-chip equipment by utilizing parylene based electrostatic microvalves. Prototypes of the system were fabricated and tested using colored DI water and 3 μm diameter micro beads, emulating drugs and cells respectively. During the tests, micro beads could be successfully entrappe...
A microarray based expression profiling of paclitaxel and vincristine resistant MCF-7 cells DEMİREL KARS, MELTEM; Iseri, Ozlem Darcansoy; Gündüz, Ufuk (2011-04-25) Resistance to the broad spectrum of chemotherapeutic agents in cancer cell lines and tumors has been called multiple drug resistance (MDR). In this study, the molecular mechanisms of resistance to two anticancer agents (paclitaxel and vincristine) in mammary carcinoma cell line MCF-7 were investigated. Drug resistant sublines to paclitaxel (MCF-7/Pac) and vincristine (MCF-7/Vinc) that were developed from sensitive MCF-7 cells (MCF-7/S) were used. cDNA microarray analysis was performed for the RNA samples of...
An Integrated imaging sensor for rare cell detection applications Altıner, Çağlar; Akın, Tayfun; Eminoğlu, Selim; Department of Micro and Nanotechnology (2012) Cell detection using image sensors is a novel and promising technique that can be used for diagnostic applications in medicine. For this purpose, cell detection studies with shadowing method are performed with yeast cells (Saccharomyces cerevisiae) using an 32×32 complementary metal oxide semiconductor (CMOS) image sensor that is sensitive to optical illumination. Cells that are placed zero distance from the sensor surface are detected using the image sensor which is illuminated with four fixed leds to main...
A two-compartment bone tumor model to investigate interactions between healthy and tumor cells Komez, Aylin; Buyuksungur, Arda; Antmen Altunsoy, Ezgi; Swieszkowski, Wojciech; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2020-01-01) We produced a novel three-dimensional (3D) bone tumor model (BTM) to study the interactions between healthy and tumor cells in a tumor microenvironment, the migration tendency of the tumor cells, and the efficacy of an anticancer drug, Doxorubicin, on the cancer cells. The model consisted of two compartments: (a) a healthy bone tissue mimic, made of poly(lactic acid-co-glycolic acid) (PLGA)/beta-tricalcium phosphate (beta-TCP) sponge seeded with human fetal osteoblastic cells (hFOB) and human umbilical vein...

Citation Formats

Y. Demircan Yalçın et al., “A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting,” Scientific Reports, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85108812547&origin=inward.