Arabidopsis C-Terminal Domain Phosphatase-Like 1 Functions in miRNA Accumulation and DNA Methylation

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2013-09-01

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Jeong, In Sil
Aksoy, Emre
Fukudome, Akihito
Akhter, Salina
Hiraguri, Akihiro
Fukuhara, Toshiyuki
Bahk, Jeong Dong
Koiwa, Hisashi

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Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated for gene regulation mediated by dsRNA-dependent pathways. We investigated the role of CPL1 and its dsRBMs in various gene silencing pathways. Genetic interaction analyses revealed that cpl1 was able to partially suppress transcriptional gene silencing and DNA hypermethylation phenotype of ros1 suggesting CPL1 is involved in the RNA-directed DNA methylation pathway without reducing siRNA production. By contrast, cpl1 reduced some miRNA levels at the level of processing. Indeed, CPL1 protein interacted with proteins important for miRNA biogenesis, suggesting that CPL1 regulates miRNA processing. These results suggest that CPL1 regulates DNA methylation via a miRNA-dependent pathway.

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https://hdl.handle.net/11511/92962

Journal

PLOS ONE

DOI

https://doi.org/10.1371/journal.pone.0074739

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Department of Biology, Article

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Citation Formats

I. S. Jeong et al., “Arabidopsis C-Terminal Domain Phosphatase-Like 1 Functions in miRNA Accumulation and DNA Methylation,” PLOS ONE, vol. 8, no. 9, pp. 0–0, 2013, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/92962.