Downregulation of imidacloprid resistant genes alters the biological parameters in Colorado potato beetle, Leptinotarsa decemlineata Say (chrysomelidae: Coleoptera)

Naqqash, Muhammad Nadir
Gokce, Ayhan
Aksoy, Emre
Bakhsh, Allah
Colorado potato beetle, Leptinotarsa decemlineata Say (coleoptera: chrysomelidae), is the important pest of potato all over the world. This insect pest is resistant to more than 50 active compounds belonging to various chemical groups. Potential of RNA interference (RNAi) was explored to knock down transcript levels of imidacloprid resistant genes in Colorado potato beetle (CPB) under laboratory conditions. Three important genes belonging to cuticular protein (CP), cytochrome P450 monoxygenases (P450) and glutathione synthetase (GSS) families encoding imidacloprid resistance were targeted. Feeding bioassays were conducted on various stages of imidacloprid resistant CPB lab population by applying HT115 expressing dsRNA on potato leaflets. Survival rate of insects exposed to CP-dsRNA decreased to 4.23%, 1532% and 47.35% in 2nd, 3rd and 4th instar larvae respectively. Larval weight and pre-adult duration were also affected due to dsRNAs feeding. Synergism of RNAi with imidacloprid conducted on the 2nd instar larvae, exhibited 100% mortality of larvae when subjected to reduced doses of GSS and CP dsRNAs along with imidacloprid. Utilization of three different dsRNAs against imidacloprid resistant CPB population reveal that dsRNAs targeting CP, P450 and GSS enzymes could be useful tool in management of imidacloprid resistant CPB populations. (C) 2019 Elsevier Ltd. All rights reserved.


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The Anacapa deer mouse is an endemic subspecies that inhabits Anacapa Island, part of Channel Islands National Park, California. We used mitochondrial DNA cytochrome c oxidase subunit II gene (COII) and 10 microsatellite loci to evaluate the levels of genetic differentiation and variation in similar to 1400 Anacapa deer mice sampled before and for 4 years after a black rat (Rattus rattus) eradication campaign that included trapping, captive holding and reintroduction of deer mice. Both mitochondrial and mic...
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Pineacea is the largest family of conifers that includes 51 species of Abies which is the second largest genus after Pinus. There are six native taxa in Turkey belonging to this genus. Four of these taxa (Abies cilicica subsp. isaurica, Abies nordmanniana subsp. bornmülleriana, Abies nordmanniana subsp. equi-trojani, Abies x olcayana) are endemic and considered as low risk (LR) species according to the IUCN criteria. To determine the phylogenetic relationship in Abies spp. in Turkey, 18 populations of diffe...
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The genus Salix L. is represented by more than 500 species in the world where 27 species are naturally found in Turkey. This genus has been commonly used as biomass production for energy, phytoremediation and pharmacological products. The objective of this study is to estimate the haplotype frequency analysis of native willow species in Turkey. The sequence data are obtained from non-coding (trnL) and coding (matK and rbcL) cpDNA gene regions of Salix species (24 species and one hybrid). Results showed that...
Durgut, Bilge; Kaya, Zeki; Department of Molecular Biology and Genetics (2022-2-23)
Salix alba and Populus nigra are two important tree species of riparian ecosystems. With a great ecologic and economic significance, they are naturally distributed in almost all river basins of Turkey. The genetic structures of these species in two river basins in Turkey were studied using eight common microsatellite markers to both species to reveal the impacts of habitat fragmentation. Therefore, the studied rivers were selected to represent a highly fragmented river, and a protected river from habitat fr...
Citation Formats
M. N. Naqqash, A. Gokce, E. Aksoy, and A. Bakhsh, “Downregulation of imidacloprid resistant genes alters the biological parameters in Colorado potato beetle, Leptinotarsa decemlineata Say (chrysomelidae: Coleoptera),” CHEMOSPHERE, vol. 240, pp. 0–0, 2020, Accessed: 00, 2021. [Online]. Available: