Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages.

Date

2021-10-06

Author

Balbasi, Emre
Sezginmert, Dersu
Alganatay, Ceren
Terzi Cizmecioglu, Nihal

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

230
views

0
downloads

The self-renewal and pluripotency features of mouse embryonic stem cells (mESCs) make them a great tool to study early mammalian development. Various signaling pathways that shape early mammalian development can be mimicked for in vitro mESC differentiation toward primitive lineages first and more specialized cell types later. Since the precise nature of the molecular mechanisms and the crosstalk between these signaling pathways is yet to be fully understood, there is a high level of variability in the efficiency and synchronicity among available differentiation protocols. Commitment of mESCs toward mesoderm, endoderm, or neuroectoderm lineages happens over only a few days and is highly efficient. Here, we provide protocols for the directed differentiation of mESCs toward these lineages in vitro.

Subject Keywords

Mouse embryonic stem cells, mESCs , mESC differentiation , Primary germ layers, Endoderm , Mesoderm, Ectoderm

URI

https://hdl.handle.net/11511/93300
https://link.springer.com/protocol/10.1007%2F7651_2021_439

DOI

https://doi.org/10.1007/7651_2021_439

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

SETD3-dependent gene expression changes during endoderm differentiation of mouse embryonic stem cells Balbaşı, Emre; Terzi Çizmecioğlu, Nihal; Department of Biology (2022-2-09) Mouse embryonic stem cells (mESCs) are pluripotent cells that have self renewal capability. They can differentiate into all three primary germ layers: mesoderm, endoderm, and ectoderm during embryonic development. The embryonic development is controlled via spatiotemporal regulation of gene expression changes. The collaborative effects of Wnt, Nodal, BMP signaling pathways help form the primitive streak, and the subsequent definitive endoderm layer in the gastrulating embryo. Deactivation of core pluripoten...
The Effect of SETD3 on Beta-Catenin and Canonical Wnt Signaling Pathway Activity in Mouse Embryonic Stem Cells Alganatay, Ceren; Terzi Çizmecioğlu, Nihal; Department of Molecular Biology and Genetics (2023-1-16) Mouse embryonic stem cells (mESCs) are self-renewing, pluripotent cells that can differentiate into endoderm, mesoderm and neuroectoderm. As differentiation requires deactivation of pluripotency factors and activation of germ layer specific genes, many epigenetic factors play an essential role. A previously performed shRNA screen showed that SETD3 which is a SET-domain containing epigenetic factor, is an essential factor for mesendoderm differentiation of mESCs. Our preliminary data demonstrated that when m...
Role of Vibrational Spectroscopy in Stem Cell Research Aksoy, Ceren; Severcan, Feride (2012-01-01) Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the ste...
Development of a UV crosslinked biodegradable hydrogel containing adipose derived stem cells to promote vascularization for skin wounds and tissue engineering Eke, Gozde; Mangir, Naside; Hasırcı, Nesrin; MacNeil, Sheila; Hasırcı, Vasıf Nejat (2017-06-01) The aim of this study was to design a dermal substitute containing adipose derived stem cells (ADSC) that can be used to improve the regeneration of skin on difficult wound beds by stimulating rapid neovascularization. This was achieved by first synthesizing methacrylated gelatin (GeIMA) and methacrylated hyaluronic acid (HAMA) precursors which could be stored at -80 degrees C after lyophilisation. Polymer precursors were then dissolved in media (in 15:1 ratio), ADSCs added together with the photoinitiator ...
Effect of scaffold architecture and BMP-2/BMP-7 delivery on in vitro bone regeneration Yilgor, Pinar; Sousa, Rui A.; Reis, Rui L.; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2010-11-01) The aim of this study was to develop 3-D tissue engineered constructs that mimic the in vivo conditions through a self-contained growth factor delivery system. A set of nanoparticles providing the release of BMP-2 initially followed by the release of BMP-7 were incorporated in poly(epsilon-caprolactone) scaffolds with different 3-D architectures produced by 3-D plotting and wet spinning. The release patterns were: each growth factor alone, simultaneous, and sequential. The orientation of the fibers did not ...

Citation Formats

E. Balbasi, D. Sezginmert, C. Alganatay, and N. Terzi Cizmecioglu, “Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages.,” 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/93300.