Individual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p

Date

2021-01-01

Author

Asim, Arslan
Gokce, Zahide Neslihan Ozturk
Bakhsh, Allah
Cayli, Ilknur Tindas
Aksoy, Emre
Csliskan, Sevgi
Caliskan, Mehmet Emin
Demirel, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

143
views

0
downloads

MicroRNAs (miRNAs) are essential players of plant defence systems because of their involvement in reprogramming gene expression under adverse environmental conditions including drought and heat, which are considered major players in limiting crop productivity. miR172b-3p was previously determined as a remarkable stress-responsive miRNA in our next-generation sequencing (NGS) analysis in potato. This study aims to understand the functions of miR172b-3p and its target (ERTF RAP2-7-like) under drought, heat, and combined treatments by overexpressing the miR172b-3p in stress-tolerant (Unica) and sensitive (Russet Burbank) potato cultivars. miR172b-3p overexpression in transgenic lines suppressed the ERTF RAP2-7-like expression leading to enhanced carbon fixation efficiency. Meanwhile, the accumulation of hydrogen peroxide (H2O2) was reduced in both cultivars, proving that it is involved in the front-line tolerance mechanism against individual drought, heat, and their combination. In conclusion, our results prove that the stress tolerance could be enhanced by miR172b-3p-mediated negative regulation of ERTF RAP2-7-like gene in potato under drought, heat, and their combination. Our findings represent the first step towards the improvement of tolerance against multiple abiotic stresses in potato.

URI

https://hdl.handle.net/11511/94276

Journal

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY

DOI

https://doi.org/10.3906/tar-2103-60

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Physiological and biochemical screeing of different Turkish Lentil (Lens culinaris M.) cultivars under drought stress condition Gökçay, Derya; Yücel, Ayşe Meral; Baloğlu, Mehmet Cengiz; Department of Biotechnology (2012) Legumes being the most important crops worldwide are limited in terms of adaptability and productivity mainly by the abiotic stresses. In this study, the aim was to understand tolerance mechanisms of lentil cultivars under drought stress by physiological and biochemical analyses. This study was carried out with six Turkish Lentil cultivars (Seyran, Kafkas, Malazgirt, Çağıl, Çiftçi, Özbek) subjected to drought stresses (10% and 15% PEG) and their physiological and biochemical properties were examined to sele...
Population variation in drought resistance and its relationship with adaptive and physiological seedling traits in Turkish red pine (Pinus brutia Ten.) Kandemir, Gaye; Önde, Sertaç; TEMEL, FATİH; Kaya, Zeki (2017-01-01) Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the re...
Genome-Wide Diversity of MADS-Box Genes in Bread Wheat is Associated with its Rapid Global Adaptability Raza, Qasim; Riaz, Awais; Atif, Rana Muhammad; Hussaın, Babar; Rana, Iqrar Ahmad; Ali, Zulfiqar; Budak, Hikmet; Alaraidh, Ibrahim A. (2022-01-01) MADS-box gene family members play multifarious roles in regulating the growth and development of crop plants and hold enormous promise for bolstering grain yield potential under changing global environments. Bread wheat (Triticum aestivum L.) is a key stable food crop around the globe. Until now, the available information concerning MADS-box genes in the wheat genome has been insufficient. Here, a comprehensive genome-wide analysis identified 300 high confidence MADS-box genes from the publicly available re...
Enhanced salt tolerance of transgenic tobacco expressing a wheat salt tolerance gene KAVAS, MUSA; BALOĞLU, MEHMET CENGİZ; Yücel, Ayşe Meral; Öktem, Hüseyin Avni (2016-01-01) Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants i...
Modeling cyanobacterial blooms in tropical reservoirs: The role of physicochemical variables and trophic interactions Alves Amorım, Cıhelıo; Dantas, Enio Wocyli; Moura, Ariadne do Nascimento (2020-11-01) Understanding the importance of environmental variables on the dominance of cyanobacteria is crucial for appropriately managing water resources. Although studies about temperate and subtropical regions show a high influence of nutrients and temperature on blooms, this relationship is still unclear for the tropics. Accordingly, we hypothesized that nutrients and temperature arc the main factors driving cyanobacterial blooms in tropical reservoirs, and those relationships are intensified by the zooplankton. T...

Citation Formats

A. Asim et al., “Individual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p,” TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, vol. 45, no. 5, pp. 651–669, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/94276.