Nanostructured modifications of titanium surfaces improve vascular regenerative properties of exosomes derived from mesenchymal stem cells: Preliminary in vitro results

Gardin, Chiara
Ferroni, Letizia
Erdoğan, Yaşar Kemal
Zanotti, Federica
De Francesco, Francesco
Trentini, Martina
Brunello, Giulia
Ercan, Batur
Zavan, Barbara
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.(1) Background: Implantation of metal-based scaffolds is a common procedure for treating several diseases. However, the success of the long-term application is limited by an insufficient endothelialization of the material surface. Nanostructured modifications of metal scaffolds represent a promising approach to faster biomaterial osteointegration through increasing of endothelial commitment of the mesenchymal stem cells (MSC). (2) Methods: Three different nanotubular Ti surfaces (TNs manufactured by electrochemical anodization with diameters of 25, 80, or 140 nm) were seeded with human MSCs (hMSCs) and their exosomes were isolated and tested with human umbilical vein endothelial cells (HUVECs) to assess whether TNs can influence the secretory functions of hMSCs and whether these in turn affect endothelial and osteogenic cell activities in vitro. (3) Results: The hMSCs adhered on all TNs and significantly expressed angiogenic-related factors after 7 days of culture when compared to untreated Ti substrates. Nanomodifications of Ti surfaces significantly improved the release of hMSCs exosomes, having dimensions below 100 nm and expressing CD63 and CD81 surface markers. These hMSC-derived exosomes were efficiently internalized by HUVECs, promoting their migration and differentiation. In addition, they selectively released a panel of miRNAs directly or indirectly related to angiogenesis. (4) Conclusions: Preconditioning of hMSCs on TNs induced elevated exosomes secretion that stimulated in vitro endothelial and cell activity, which might improve in vivo angiogenesis, supporting faster scaffold integration.


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© 2021 by the authors. Licensee MDPI, Basel, Switzerland.Meso-substituted borondipyrromethene (BODIPY)-porphyrin compounds that include free base porphyrin with two different numbers of BODIPY groups (BDP-TTP and 3BDP-TTP) were designed and synthesized to analyze intramolecular energy transfer mechanisms of meso-substituted BODIPY-porphyrin dyads and the effect of the different numbers of BODIPY groups connected to free-base porphyrin on the energy transfer mechanism. Absorption spectra of BODIPY-porphyrin ...
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland.Fluctuations in fossil fuel prices significantly affect the economies of countries, especially oil-importing countries, hence these countries are thoroughly investigating the increase in the utilization of renewable energy resources as it is abundant and locally available in all the countries despite challenges. Renewable energy systems (RES) such as solar and wind systems offer suitable alternatives for fossil fuels and could ensure the energy securi...
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Citation Formats
C. Gardin et al., “Nanostructured modifications of titanium surfaces improve vascular regenerative properties of exosomes derived from mesenchymal stem cells: Preliminary in vitro results,” Nanomaterials, vol. 11, no. 12, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: