Nanostructured modifications of titanium surfaces improve vascular regenerative properties of exosomes derived from mesenchymal stem cells: Preliminary in vitro results

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nanomaterials-11-03452-v2.pdf

Date

2021-12-01

Author

Gardin, Chiara
Ferroni, Letizia
Erdoğan, Yaşar Kemal
Zanotti, Federica
De Francesco, Francesco
Trentini, Martina
Brunello, Giulia
Ercan, Batur
Zavan, Barbara

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© 2021 by the authors. Licensee MDPI, Basel, Switzerland.(1) Background: Implantation of metal-based scaffolds is a common procedure for treating several diseases. However, the success of the long-term application is limited by an insufficient endothelialization of the material surface. Nanostructured modifications of metal scaffolds represent a promising approach to faster biomaterial osteointegration through increasing of endothelial commitment of the mesenchymal stem cells (MSC). (2) Methods: Three different nanotubular Ti surfaces (TNs manufactured by electrochemical anodization with diameters of 25, 80, or 140 nm) were seeded with human MSCs (hMSCs) and their exosomes were isolated and tested with human umbilical vein endothelial cells (HUVECs) to assess whether TNs can influence the secretory functions of hMSCs and whether these in turn affect endothelial and osteogenic cell activities in vitro. (3) Results: The hMSCs adhered on all TNs and significantly expressed angiogenic-related factors after 7 days of culture when compared to untreated Ti substrates. Nanomodifications of Ti surfaces significantly improved the release of hMSCs exosomes, having dimensions below 100 nm and expressing CD63 and CD81 surface markers. These hMSC-derived exosomes were efficiently internalized by HUVECs, promoting their migration and differentiation. In addition, they selectively released a panel of miRNAs directly or indirectly related to angiogenesis. (4) Conclusions: Preconditioning of hMSCs on TNs induced elevated exosomes secretion that stimulated in vitro endothelial and cell activity, which might improve in vivo angiogenesis, supporting faster scaffold integration.

Subject Keywords

Angiogenesis, Cardiovascular metal stents, Endothelial cells, Endothelialization, Mesenchymal stem cells, Titanium nanotubes

URI

https://hdl.handle.net/11511/95039

Journal

Nanomaterials

DOI

https://doi.org/10.3390/nano11123452

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

C. Gardin et al., “Nanostructured modifications of titanium surfaces improve vascular regenerative properties of exosomes derived from mesenchymal stem cells: Preliminary in vitro results,” Nanomaterials, vol. 11, no. 12, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/95039.