Establishment of Human Induced Pluripotent Stem Cells from Multiple Sclerosis Patients.

Date

2022-01-06

Author

Begentaş, Onur Can
Koc, Dilara
Kiris, Erkan

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The patient-derived iPSC lines provide valuable resources as these cells can be utilized to generate human cell types relevant to the disease of interest. In this context, human iPSC-based model systems are particularly useful for neurological diseases as the neuron and glial cell types affected by such diseases are difficult to obtain. Multiple sclerosis is a demyelinating central nervous system disease characterized by inflammation and eventually axonal damage. iPS cells generated from MS patients may allow for unique approaches for studying the disease in a species-specific manner, with a potentially limitless supply of patients’ own glial and neuronal cells differentiated from the iPSCs. Here we describe the detailed protocol for establishing iPSCs from peripheral blood mononuclear cells that we have utilized to model multiple sclerosis. We particularly focused on optimized and cost-effective procedures using the integration-free Sendai virus-based reprogramming method for the generation and characterization of MS iPSCs.

Subject Keywords

Disease modeling, Human induced pluripotent stem cells, Multiple sclerosis, Pluripotency , Sendai virus-based reprogramming

URI

https://hdl.handle.net/11511/96024

Journal

Methods in molecular biology (Clifton, N.J.)

DOI

https://doi.org/10.1007/7651_2021_453

Collections

Department of Biology, Article

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Citation Formats

O. C. Begentaş, D. Koc, and E. Kiris, “Establishment of Human Induced Pluripotent Stem Cells from Multiple Sclerosis Patients.,” Methods in molecular biology (Clifton, N.J.), 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/96024.