Identification of protein partners of setd3 in mouse embryonic stem cells

Güven, Gözde
Embryonic development is a highly coordinated process that contains rapid cell divisions. During this time, the cells also start differentiation, and at the end of development, the cells become fully differentiated. This differentiation commitment is regulated by epigenetic factors like histone modifiers, chromatin regulators. SETD3 is one of the histone methyltransferases that govern this pluripotency-differentiation balance. We have previously found that SETD3 is crucial for mesendoderm differentiation of mESCs. However, the partners of Setd3 in the nucleus is not well known. We have performed IP-MS and analyzed the candidate proteins deeply. Then, we performed biochemical assays (co-IP and PLA) and showed BRD2 and TFCP2L1 proteins are the protein partners of SETD3 methyltransferase.


Identification of transcription factors that interact with Arid4b protein in mouse embryonic stem cells
Keskin, Ezgi Gül; Terzi Çizmecioğlu, Nihal; Department of Molecular Biology and Genetics (2019)
Embryonic development is a process that is highly regulated at the level of gene expression. Throughout this process, the zygote divides and differentiates to form all cell types in the adult body. In the early stages of development, pluripotent embryonic stem cells (ESC) can be isolated and used to study cell fate mechanisms during development in vitro. The pluripotency of ESCs is governed by certain transcription factors (TF) (Oct4, Nanog, etc.). These TFs construct a transcription network specific to ESC...
Arid4b alters cell cycle and cell death dynamics during mouse embryonic stem cell differentiation.
Güven, Gözde; Terzi Çizmecioğlu, Nihal (2021-02-09)
Cell division and death play an important role in embryonic development. Cell specialization is accompanied with slow proliferation and quiescence. Cell death is important for morphogenesis. Gene expression changes during differentiation is coordinated by lineage-specific transcription factors and chromatin factors. It is not yet fully understood how alterations in gene expression and cell cycle/death mechanisms are connected. We previously identified a chromatin protein Arid4b as a critical factor for meso...
Establishment of Human Induced Pluripotent Stem Cells from Multiple Sclerosis Patients.
Begentaş, Onur Can; Koc, Dilara; Kiris, Erkan (2022-01-06)
The patient-derived iPSC lines provide valuable resources as these cells can be utilized to generate human cell types relevant to the disease of interest. In this context, human iPSC-based model systems are particularly useful for neurological diseases as the neuron and glial cell types affected by such diseases are difficult to obtain. Multiple sclerosis is a demyelinating central nervous system disease characterized by inflammation and eventually axonal damage. iPS cells generated from MS patients may all...
Role of Vibrational Spectroscopy in Stem Cell Research
Aksoy, Ceren; Severcan, Feride (2012-01-01)
Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the ste...
Characterization and identification of human mesenchymal stem cells at molecular level
Aksoy, Ceren; Severcan, Feride; Çetinkaya, Duygu Uçkan; Department of Biotechnology (2012)
Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...
Citation Formats
G. Güven, “Identification of protein partners of setd3 in mouse embryonic stem cells,” M.S. - Master of Science, Middle East Technical University, 2021.