Assessment of antimicrobial and coagulant activities of CBRN decontamination materials

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2022-2

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Tosun, Barış Çağrı

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CBRN Defense is an emerging interdisciplinary branch of science that combines physics, chemistry and biology together in a unique and complementary way. Concept of decontamination, which is a necessity to neutralize or reduce the spread of contaminants, is one of the key topics in this area of study, and many of the commercially available products are in liquid form and mainly intended to be used for decontamination of environment and equipments. Material used in this study is in powder form that is not dissolvable in liquids, and thus providing a novel procedure for testing antimicrobial effect of such materials as well. Decon powder is intended to be used on human skin for decontamination against CBRN agents, and its effect on blood coagulation for superficial bleedings was also investigated. We applied varying doses of decon powder on E.coli, B.subtilis and B.subtilis endospores and also along with another known antibacterial compound, protamine, to determine the minimum concentration of powder needed to prevent the visible growth of colonies on agar plates, and found that Gram positive bacteria are more easily affected by the decon powder formulation compared to Gram negative bacteria. Minimum Inhibitory concentration for B.subtilis was determined to be 50 mg decon powder, whereas for E.coli, it was 100 mg given 60 minutes, and 500 mg given 15 minutes. Decon powder formulation could prevent the activation and significantly reduce the visible growth of endospores with 500 mg dose and 100% reduction in growth was observed at 200 mg decon powder mixture with 50 mg protamine. Furthermore, using whole blood sample, we determined that the application of decon powder could also help reducing the superficial bleedings in vitro compared to standard gauzes, and this effect was found statistically significant.

Subject Keywords

CBRN, Decontamination, CFU, Antibacterial, Coagulation

URI

https://hdl.handle.net/11511/96082

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

B. Ç. Tosun, “Assessment of antimicrobial and coagulant activities of CBRN decontamination materials,” M.S. - Master of Science, Middle East Technical University, 2022.