COMPARISON OF DIFFERENT ANTIOXIDANT ASSAYS FOR ESTIMATION OF ANTIOXIDANT POTENTIAL OF SELECTED FRUIT JUICE WASTE MATERIALS

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2022-2-11

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Gülenç, Barış Ege

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Fruit juice industry waste utilization includes several options that one of them extraction of bioactive compounds exhibiting antioxidant effect. In this study, ultrasound assisted extraction was applied to dried apple, sour cherry and grape pomaces by using ethanol, 50% aqueous ethanol and water solvents. For antioxidant capacity estimation of the extracts, DPPH•, ABTS•+, CUPRAC and FRAP assays were applied. The highest total phenolic contents were obtained for sour cherry and grape pomace 50% aqueous ethanol extracts where the highest total phenolic content for apple pomace was obtained by water extraction. According to the FRAP method, the highest antioxidant capacities were obtained by 50% aqueous ethanol extraction and grape pomace extracts provided the highest antioxidant capacity. Similarly, the highest antioxidant capacity was determined for the grape pomace 50% aqueous ethanol extract according to CUPRAC assay. According to DPPH• method, 50% aqueous ethanol pomace extracts provided the highest antioxidant capacity with lowest EC20 value and the apple pomace extract provided the highest antioxidant capacity. According to the ABTS•+ EC20 values, 50% aqueous ethanol extracts for grape and apple pomaces provided the highest antioxidant capacity. FRAP and CUPRAC values were significantly (p< 0.01) correlated with total phenolic contents. Significant correlation between CUPRAC and FRAP assays was also obtained (p<0.01). Low correlations were obtained between DPPH• EC20 and metal ion reducing capacity assays. The correlation was much better between ABTS•+ EC50 and FRAP assays especially for sour cherry pomace.

Subject Keywords

fruit pomace, phenolic content, antioxidant capacity

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https://hdl.handle.net/11511/96332

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

B. E. Gülenç, “COMPARISON OF DIFFERENT ANTIOXIDANT ASSAYS FOR ESTIMATION OF ANTIOXIDANT POTENTIAL OF SELECTED FRUIT JUICE WASTE MATERIALS,” M.S. - Master of Science, Middle East Technical University, 2022.