COMPARISON OF DIFFERENT ANTIOXIDANT ASSAYS FOR ESTIMATION OF ANTIOXIDANT POTENTIAL OF SELECTED FRUIT JUICE WASTE MATERIALS

Download

index.pdf

Date

2022-2-11

Author

Gülenç, Barış Ege

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

534
views

691
downloads

Fruit juice industry waste utilization includes several options that one of them extraction of bioactive compounds exhibiting antioxidant effect. In this study, ultrasound assisted extraction was applied to dried apple, sour cherry and grape pomaces by using ethanol, 50% aqueous ethanol and water solvents. For antioxidant capacity estimation of the extracts, DPPH•, ABTS•+, CUPRAC and FRAP assays were applied. The highest total phenolic contents were obtained for sour cherry and grape pomace 50% aqueous ethanol extracts where the highest total phenolic content for apple pomace was obtained by water extraction. According to the FRAP method, the highest antioxidant capacities were obtained by 50% aqueous ethanol extraction and grape pomace extracts provided the highest antioxidant capacity. Similarly, the highest antioxidant capacity was determined for the grape pomace 50% aqueous ethanol extract according to CUPRAC assay. According to DPPH• method, 50% aqueous ethanol pomace extracts provided the highest antioxidant capacity with lowest EC20 value and the apple pomace extract provided the highest antioxidant capacity. According to the ABTS•+ EC20 values, 50% aqueous ethanol extracts for grape and apple pomaces provided the highest antioxidant capacity. FRAP and CUPRAC values were significantly (p< 0.01) correlated with total phenolic contents. Significant correlation between CUPRAC and FRAP assays was also obtained (p<0.01). Low correlations were obtained between DPPH• EC20 and metal ion reducing capacity assays. The correlation was much better between ABTS•+ EC50 and FRAP assays especially for sour cherry pomace.

Subject Keywords

fruit pomace, phenolic content, antioxidant capacity

URI

https://hdl.handle.net/11511/96332

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste Attard, Karen; Öztop, Halil Mecit; Lia, Frederick (2022-12-01) This study presents the effect of hydrolysis on the antioxidant activity of olive mill waste. The olive pomace samples were collected at different stages of maturity and were investigated for their phenolic content and antioxidant activity. Three different extraction procedures were employed, including methanolic maceration extraction and two hydrolysed procedures using 6 M HCL for acid hydrolysis and 10 M NaOH for alkaline hydrolysis. The total phenolic, flavonoid and ortho-diphenolic content, metal ion re...
Comparison of physicochemical characteristics and photofermentative hydrogen production potential of wastewaters produced from different olive oil mills in Western-Anatolia, Turkey Eroglu, Ela; Eroglu, Inci; Gündüz, Ufuk; Yuecel, Meral (2009-04-01) Olive oil extraction produces a dark-colored wastewater that contains nutrients that can be further processed using biotechnology, in parallel with treatment for disposal. For instance, olive mill wastewater (OMW) can be used as a substrate for photofermentative hydrogen production by purple bacteria. A comparative study was investigated with several OMW samples front different olive oil mills in Western-Anatolia, Turkey. The composition of OMW varies significantly for each mill; thus, a detailed physicoche...
Determination of phenolic compound profiles and antioxidant effect of black elderberry, echinacea and iceland moss extracts on late-release soft lozenge Yıldız, Özge; Bayındırlı, Alev; Çapanoğlu Güven, Esra; Department of Food Engineering (2021-6) Medicinal plants contain different bioactive compounds that have immune system stimulant, anti-inflammatory, antibacterial, antiviral, antifungal, anticancer and wound healing effects. Echinacea purpurea L., Sambucus nigra L. and Cetraria islandica L. are known with their high antioxidant content and health promoting properties. The main objective of the study was to determine the total phenolic content, total flavonoid content and antioxidant capacity of each plant extract with water and 75 % ethanol as so...
Comparison of microwave and ultrasound-assisted extraction techniques for leaching of phenolic compounds from nettle Ince, Alev Emine; Şahin, Serpil; Şümnü, Servet Gülüm (2014-10-01) In this study, extraction of phenolic compounds from nettle by microwave and ultrasound was studied. In both microwave and ultrasound-assisted extractions, effects of extraction time (5-20 min for microwave; 5-30 min for ultrasound) and solid to solvent ratio (1:10, 1:20, and 1:30 g/mL) on total phenolic content (TPC) were investigated. Effects of different powers (50 % and 80 %) were also studied for ultrasound-assisted extraction. In microwave-assisted extraction, the optimum TPC of the extracts (24.64 +/...
Optimisation of antioxidant activity of grape cane extracts using response surface methodology Karacabey, Erkan; Mazza, Giuseppe (2010-03-01) Solid-liquid extraction and response surface methodology were used to optimise conditions for the antioxidant activity of grape cane extracts. The independent processing variables were ethanol concentration, temperature and solvent to solid ratio. Ethanol concentration and temperature significantly affected antioxidant activity measured by the Trolox equivalent antioxidant capacity (TEAC) assay and the oxygen radical absorbance capacity using fluorescein (ORAC(FL)) method (p 0.05). Antioxidant activity of ...

Citation Formats

B. E. Gülenç, “COMPARISON OF DIFFERENT ANTIOXIDANT ASSAYS FOR ESTIMATION OF ANTIOXIDANT POTENTIAL OF SELECTED FRUIT JUICE WASTE MATERIALS,” M.S. - Master of Science, Middle East Technical University, 2022.