Determination of phenolic compound profiles and antioxidant effect of black elderberry, echinacea and iceland moss extracts on late-release soft lozenge

Download

Özge Yıldız_Thesis_23.06.2021.pdf

Date

2021-6

Author

Yıldız, Özge

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

605
views

754
downloads

Medicinal plants contain different bioactive compounds that have immune system stimulant, anti-inflammatory, antibacterial, antiviral, antifungal, anticancer and wound healing effects. Echinacea purpurea L., Sambucus nigra L. and Cetraria islandica L. are known with their high antioxidant content and health promoting properties. The main objective of the study was to determine the total phenolic content, total flavonoid content and antioxidant capacity of each plant extract with water and 75 % ethanol as solvents. The extraction efficiency was significantly higher in ethanol extracts according to total phenolic content, total flavonoid content, 1-diphenyl-2-picryl-hydrazyl (DPPH) and copper reducing antioxidant capacity (CUPRAC) analysis (p<0.05). Among all extracts, the highest result of radical scavenging capacity was found in ethanol extract of Sambucus nigra L. as 901.62±24.53 mg TEAC/ g dry extract. Total flavonoid and total phenolic contents of the same extract were determined as 840.54±13.46 mg RE/ g dry extract and 339.68±1.47 mg GAE/ g dry extract, respectively. The selected phenolic compounds were quantified and phenolic profiles were determined by HPLC analysis for all extracts. By using the selected plant powders, the late-release soft lozenge product was formulated. Loss of bioactive compounds was examined by using spectrophotometric and chromatographic techniques. In lozenge product, the highest phenolic content was obtained with Sambucus nigra L. (2.33±0.11 mg GAE/ g dry extract. Results confirmed that the lozenge application caused a significant loss in antioxidant amount for all plant extracts. In order to obtain optimum antioxidant effectiveness from the lozenge products with functional properties, it is needed to provide a suitable recipe with optimized usage of plant extracts.

Subject Keywords

Phenolic Content, Antioxidant Capacity, Medicinal Plant, Soft Lozenge

URI

https://hdl.handle.net/11511/91435

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

EVALUATION OF IN VIVO EFFECTS OF VISCUM ALBUM L. AND ITS CONSTITUENT O-COUMARIC ACID ON BILE ACID SYNTHESIZING CYPS Ege, Tuba; Ozkan, Ayse Mine Gencler; Sena, Alaattin; Adalı, Orhan (2019-01-01) This research was designed to determine about the possible potency of medicinal plants Viscum album L. and its major polyphenolic ingredient, o-coumaric acid on rat liver cholesterol and bile acid metabolizing enzymes. Bioactive compounds of V. album extracts were identified using LC-MS technique. The water extracts of V. album (10 mg/kg) and o-coumaric acid (30 mg/kg) were injected intraperitoneally for 9 days. Then, in vivo effects of this plant and its major phenolic ingredient (o-coumaric acid) on rat l...
EVALUATION OF ANTIBACTERIAL AND CARBONIC ANHYDRASE INHIBITORY POTENTIAL OF METHANOLIC EXTRACT OF &ITNARDOSTACHYS JATAMANSI&IT (D. DON) DC RHIZOMES Rehman, Tayyeba; Ahmad, Saeed; Abbasi, Waheed Mumtaz; Ahmad, Ashfaq; Bilal, Muhammad; Zaman, Muhammad Mohsin; Ghauri, Aymen Owais; Arshad, Adeel; Akhtar, Khalid (2017-09-01) Many antimicrobial drugs are going to become resistant to different pathogens, so the discovery of new antimicrobial products is an important public health concern. Nardostachys jatamansi (D. Don) DC (Valerianaceae) is an important traditional herbal medicine used as tranquillizer. CNS sedative. antiepilepsy, cardiotonic. diuretic, heptatonic, analgesic and in boils, itch and eye diseases. Carbonic anhydrase inhibitors are the potential source of treatment in case of glaucoma, hypertension and epilepsy and ...
Antioxidant and cytotoxic properties of Salvia fruticosa M. and its effects on gene expressions of some CYP450 and antioxidant enzymes in HT-29 cell line Altay, Ahmet; Bozoğlu, Faruk; Tokuşoğlu, Özlem; Department of Biochemistry (2015) Salvia genus is a widely cultivated as a perennial herb and used in medicine for various purposes due to its antimicrobial, antioxidant, anticarcinogen and anti-inflammatory properties. In this study, the phenolic composition of water extract of Salvia fruticosa and possible effects of those constituents in cancer related drug metabolizing enzymes are investigated. S. fruticosa extract displayed high Radical Scavenging Activity against DPPH and ABTS radicals and also showed high Fe2+ Chelating Activity . To...
COMPARISON OF DIFFERENT ANTIOXIDANT ASSAYS FOR ESTIMATION OF ANTIOXIDANT POTENTIAL OF SELECTED FRUIT JUICE WASTE MATERIALS Gülenç, Barış Ege; Bayındırlı, Alev; Department of Food Engineering (2022-2-11) Fruit juice industry waste utilization includes several options that one of them extraction of bioactive compounds exhibiting antioxidant effect. In this study, ultrasound assisted extraction was applied to dried apple, sour cherry and grape pomaces by using ethanol, 50% aqueous ethanol and water solvents. For antioxidant capacity estimation of the extracts, DPPH•, ABTS•+, CUPRAC and FRAP assays were applied. The highest total phenolic contents were obtained for sour cherry and grape pomace 50% aqueous eth...
Studies of enzymes that cause resistance to aminoglycosides antibiotics. Serpersu, Engin H; Özen, Can; Wright, Edward (2008-01-01) Aminoglycoside antibiotics are highly potent, wide-spectrum bactericidals (1, 2). Bacterial resistance to aminoglycosides, however, is a major problem in the clinical use of aminoglycosides. Enzymatic modification of aminoglycosides is the most frequent resistance mode among several resistance mechanisms employed by resistant pathogens (1,3). Three families of aminoglycoside modifying enzymes, O-phosphotransferases, N-acetyltransferases, and N-nucleotidyltransferases, are known to have more than 50 enzymes ...

Citation Formats

Ö. Yıldız, “Determination of phenolic compound profiles and antioxidant effect of black elderberry, echinacea and iceland moss extracts on late-release soft lozenge,” M.S. - Master of Science, Middle East Technical University, 2021.