Determination of phenolic compound profiles and antioxidant effect of black elderberry, echinacea and iceland moss extracts on late-release soft lozenge

2021-6
Yıldız, Özge
Medicinal plants contain different bioactive compounds that have immune system stimulant, anti-inflammatory, antibacterial, antiviral, antifungal, anticancer and wound healing effects. Echinacea purpurea L., Sambucus nigra L. and Cetraria islandica L. are known with their high antioxidant content and health promoting properties. The main objective of the study was to determine the total phenolic content, total flavonoid content and antioxidant capacity of each plant extract with water and 75 % ethanol as solvents. The extraction efficiency was significantly higher in ethanol extracts according to total phenolic content, total flavonoid content, 1-diphenyl-2-picryl-hydrazyl (DPPH) and copper reducing antioxidant capacity (CUPRAC) analysis (p<0.05). Among all extracts, the highest result of radical scavenging capacity was found in ethanol extract of Sambucus nigra L. as 901.62±24.53 mg TEAC/ g dry extract. Total flavonoid and total phenolic contents of the same extract were determined as 840.54±13.46 mg RE/ g dry extract and 339.68±1.47 mg GAE/ g dry extract, respectively. The selected phenolic compounds were quantified and phenolic profiles were determined by HPLC analysis for all extracts. By using the selected plant powders, the late-release soft lozenge product was formulated. Loss of bioactive compounds was examined by using spectrophotometric and chromatographic techniques. In lozenge product, the highest phenolic content was obtained with Sambucus nigra L. (2.33±0.11 mg GAE/ g dry extract. Results confirmed that the lozenge application caused a significant loss in antioxidant amount for all plant extracts. In order to obtain optimum antioxidant effectiveness from the lozenge products with functional properties, it is needed to provide a suitable recipe with optimized usage of plant extracts.

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Citation Formats
Ö. Yıldız, “Determination of phenolic compound profiles and antioxidant effect of black elderberry, echinacea and iceland moss extracts on late-release soft lozenge,” M.S. - Master of Science, Middle East Technical University, 2021.