THE EFFECT OF NANOSCALE SURFACE TOPOGRAPHY ON ADHESION, PROLIFERATION AND PROTEIN EXPRESSION OF NEURONAL CELLS

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2022-3-30

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Mimiroğlu, Didem

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Neural guidance channels (NGCs) are tubular structures that are placed between distal and proximal ends of a damaged nerve to support the regeneration process. NGCs should support neural cell functions, conduct electrical impulses and have biomimetic surface characteristics. One of the approaches to attain these properties for NGCs properties is to use nanotechnology, which refers to altering physical and chemical properties of a material by controlling its size below 100 nm. There are various studies about the effects of submicron and micron sized surface features on the proliferation and biological functions of neural cells. However, studies concerning electrically conductive materials having surface features below 100nm are limited. For this reason, poly (lactic-co-glycolic) acid (PLGA), a currently-used material to fabricate NGCs, and a more accessible natural material, silk fibroin, were used to systematically obtain nanotopographical structures on their surfaces. This thesis provided data to the literature by comparing biological properties of PLGA films having 30 nm and 80 nm surface feature sizes. In addition, carbon nanofibres (CNFs) were incorporated as a secondary phase into silk fibroin films having nano and sub-micron level surface features (50 nm and 125 nm) to provide electrical conductivity, then these surfaces were tested for proliferation, viability, functions and morphologies of neural cells using neuroblastoma cell line, N2a. Findings from this thesis aimed to pave the way for future NGCs to regenerate damaged neural tissue in patients having neural tissue injury.

Subject Keywords

nanostructure, neural cells, N2a, surface topography, conductivity

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https://hdl.handle.net/11511/96811

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

D. Mimiroğlu, “THE EFFECT OF NANOSCALE SURFACE TOPOGRAPHY ON ADHESION, PROLIFERATION AND PROTEIN EXPRESSION OF NEURONAL CELLS,” Ph.D. - Doctoral Program, Middle East Technical University, 2022.