Regulation of Cytoskeletal Dynamics during T Cell Activation by Substrate Stiffness

Date

2018-03-07

Author

Özçelikkale, Altuğ
Upadhyaya, Arpita

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

120
views

0
downloads

T lymphocytes are an integral part of the adaptive immune response. The detection of infectious agents critically depends on the interaction of T cells with antigen presenting cells, which have varying mechanical stiffness and complex topological features. It has been recently recognized that T cell activation is regulated both by stiffness of the antigen presenting surface and by cytoskeletal forces which partially arise from actomyosin contractility. However, the relationship between stiffness and the force generating machinery driving T cell activation is not well understood. To address this problem, we characterized actin and myosin dynamics during the activation of Jurkat T cells on stimulatory elastic substrates with variable stiffness using total internal reflection fluorescence (TIRF) and confocal microscopy. Activated T cells exhibit lamellipodial actin and myosin flows at the cell periphery as well as lamellar rings of actomyosin bundles. We have explored the stiffness-dependent organization of these distinct actomyosin structures and flows and their correlation with the spatiotemporal variation of traction stresses. This study provides insight into the potential role of stiffness in regulating cytoskeletal organization and force generation during T cell activation.

URI

https://hdl.handle.net/11511/97062

Conference Name

American Physical Society March Meeting

Collections

Department of Mechanical Engineering, Conference / Seminar

Suggestions

OpenMETU
Core

Enhanced immunostimulatory activity of cyclic dinucleotides on mouse cells when complexed with a cell-penetrating peptide or combined with CpG Yildiz, Soner; Alpdundar, Esin; Gungor, Bilgi; Kahraman, Tamer; Bayyurt, Banu; GÜRSEL, İHSAN; Gürsel, Mayda (2015-04-01) Recognition of pathogen-derived nucleic acids by immune cells is critical for the activation of protective innate immune responses. Bacterial cyclic dinucleotides (CDNs) are small nucleic acids that are directly recognized by the cytosolic DNA sensor STING (stimulator of IFN genes), initiating a response characterized by proinflammatory cytokine and type I IFN production. Strategies to improve the immune stimulatory activities of CDNs can further their potential for clinical development. Here, we demonstrat...
Activation of survival pathways in nutrient restricted colorectal cancer cells Oral, Göksu; Banerjee, Sreeparna; Department of Biology (2023-1-9) Limited nutrient availability in the tumor microenvironment can cause metabolic rewiring of cancer cells, resulting in the activation of various stress response pathways such as autophagy for survival. Our study showed for the first time that incubation of LoVo cells with a nutrient restriction medium containing low glucose, glutamine, and serum for 48 h resulted in the concurrent activation of two antagonistic proteins: the AMP Kinase pathway (AMPK) which is phosphorylated in response to low energy and act...
Effect of cortex stiffness variation on cleavage in animal cells AKKAS, N; KERMANIAN, M (1993-09-29) Cytokinesis is the division of the cytoplasm and the plasma membrane into two. Nuclear division and cytokinesis take a relatively short time to happen in animal cells in which cytokinesis takes place by means of furrowing. Here we consider the cleavage of the animal cells only. Although the basic division mechanisms appear to be similar in all animal cells, the divisions that take place during early embryonic development, which are termed as cleavages, are not exactly the same as those that occur later. In ...
REGULATION OF M2-TYPE PYRUVATE-KINASE FROM HUMAN MENINGIOMA BY ALLOSTERIC EFFECTORS FRUCTOSE 1,6 DIPHOSPHATE AND L-ALANINE MELLATI, AA; YUCEL, M; ALTINORS, N; Gündüz, Ufuk (1992-01-01) In the present study the mechanism of action of M2-type pyruvate kinase from human meningioma in the simultaneous presence of fructose 1,6 diphosphate and L-alanine was investigated. Purified pyruvate kinase from human meningioma was allosterically inhibited by L-alanine with respect to substrates phosphoenolpyruvate and ADP. The inhibitory effects of L-alanine was partially removed by fructose 1,6 diphosphate. The purified enzyme was slightly susceptible to ATP inhibition.
Effect of protein aggregation in the aqueous phase on the binding of membrane proteins to membranes Doebler, R; Basaran, N; Goldston, H; Holloway, PW (Elsevier BV, 1999-02-01) Analysis of the binding of hydrophobic peptides or proteins to membranes generally assumes that the solute is monomeric in both the aqueous phase and the membrane. Simulations were performed to examine the effect of solute self-association in the aqueous phase on the binding of monomeric solute to lipid vesicles. Aggregation lowered the initial concentration of monomeric solute, which was then maintained at a relatively constant value at the expense of the aggregated solute, as the lipid concentration was i...

Citation Formats

A. Özçelikkale and A. Upadhyaya, “Regulation of Cytoskeletal Dynamics during T Cell Activation by Substrate Stiffness,” presented at the American Physical Society March Meeting, California, Amerika Birleşik Devletleri, 2018, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/97062.