Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Electrochemical detection of oxaliplatin induced DNA damage in G-quadruplex structures
Download
10476664.pdf
Date
2022-7
Author
Osmanoğulları, Sıla Can
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
107
views
62
downloads
Cite This
Oxaliplatin is an anticancer agent used in chemotherapy. As a platinum-based chemotherapeutic agent, it is known to induce DNA damage by generating intra- and inter-strand crosslinking mainly at N7 sites of adenine (A) or guanine (G) bases. Thus, a high dosage of Oxaliplatin results in different side effects. In order to understand the molecular mechanisms underlying these side effects and the drug resistance developing against Oxaliplatin, there is a need for rapid qualitative and quantitative determination of Oxaliplatin and the damage caused by it. Electrochemical based methods are one of the sensing platforms that can be preferred due to their sensitivity, simplicity and low cost. In this study, an electroanalytical platform for the detection of DNA damage caused by Oxaliplatin was constructed via using differential pulse voltammetry on gold nanoparticle (Au-NP)-modified graphite electrode. The surface characterization of the prepared electrodes was performed by scanning electron microscopy. The decrease in the intensity of the guanine oxidation signal with increasing Oxaliplatin concentration was taken as an indication of the binding of Oxaliplatin to DNA bases and used in the development of the detection platform with the dynamic range of 1.0 μM to 10.0 μM. The Oxaliplatin induced damage could be detected as low as at 1.0 μM Oxaliplatin concentration under the optimized conditions. These results in here are expected to offer new insights into the investigations of DNA-Oxaliplatin interactions in the future studies.
Subject Keywords
Oxaliplatin
,
DNA damage
,
Electrochemical detection
,
G-quadruplex structures
,
Differential pulse voltammetry
URI
https://hdl.handle.net/11511/98179
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Effect of gemcitabine and retinoic acid loaded PAMAM dendrimer-coated magnetic nanoparticles on pancreatic cancer and stellate cell lines
Yalcin, Serap; Erkan, Mert; Unsoy, Gozde; Parsian, Maryam; Kleeff, Jorg; Gündüz, Ufuk (2014-07-01)
Gemcitabine is an anticancer drug used in the treatment of different cancer types, including pancreatic ductal adenocarcinoma. The maximum tolerated dose in humans is restricted by its side effects on healty cells. Furthermore, the fibrotic stroma produced by the pancreatic stellate cells prevents effective delivery of chemotherapeutic agents providing a safe-haven for the cancer cells. This becomes more of a problem considering the short half-life of this drug. Magnetic nanoparticle-based targeted drug del...
Acyl chain length and charge effect on Tamoxifen-lipid model membrane interactions
Bilge, Duygu; KAZANCI, NADİDE; Severcan, Feride (Elsevier BV, 2013-05-22)
Tamoxifen (TAM), which is an antiestrogenic agent, is widely used during chemotherapy of breast, pancreas, brain and liver cancers. In this study, TAM and model membrane interactions in the form of multilamellar vesicles (MLVs) were studied for lipids containing different acyl chain length and different charge status as a function of different TAM (1, 6, 9 and 15 mol%) concentrations. Zwitterionic lipids namely dipalmitoyl phosphatidylcholine (DPPC), and dimyristoylphosphatidylcholine (DMPC) lipids were use...
Vimentin silencing effect on invasive and migration characteristics of doxorubicin resistant MCF-7 cells
Tezcan, Okan; Gündüz, Ufuk (2014-04-01)
Chemotherapy is one of the well-known treatments in cancer therapy. The effectiveness of chemotherapy is limited by several factors one of which is the emergence of multidrug resistance (MDR). One of the major mechanisms of MDR is the activity of several ATP binding cassette (ABC) transporters that pump drugs out of the cells. Doxorubicin intercalates and inhibits DNA replication; it is a powerful chemotherapeutic agent. However, it causes development of MDR in tumor cells. Vimentin is a type III intermedia...
Nanoparticles Based Drug Delivery Systems to Overcome Multidrug Resistance in Cancer The Role of Membrane Lipids Proteins and Carbohydrates
Özlüer, Özlem; Yalçın Azarkan, Serap; Gündüz, Ufuk (null; 2016-09-28)
Multidrug resistance (MDR) is a major problem in success of cancer chemotherapy on tumor cell growth, limits the prolonged and effective use of chemotherapy. The use of nanomaterial based drug carriers in cancer treatment offers exciting opportunities to enhance delivery of therapeutics to the tumor site (1). This is also known as targeted drug delivery providing differential distribution of drugs to the tumor site while significantly reducing the overall toxicity. Here, we critically discuss the role of th...
Co-delivery of Doxorubicin and D-alpha-Tocopherol Polyethylene Glycol 1000 Succinate by Magnetic Nanoparticles
Metin, Esra; Mutlu, Pelin; Gündüz, Ufuk (2018-01-01)
Background: Although conventional chemotherapy is the most common method for cancer treatment, it has several side effects such as neuropathy, alopecia and cardiotoxicity. Since the drugs are given to body systemically, normal cells are also affected, just like cancer cells. However, in recent years, targeted drug delivery has been developed to overcome these drawbacks.
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
S. C. Osmanoğulları, “Electrochemical detection of oxaliplatin induced DNA damage in G-quadruplex structures,” M.S. - Master of Science, Middle East Technical University, 2022.