Vancomycin Containing PDLLA and PLGA/β-TCP Inhibit Biofilm Formation but Do Not Stimulate Osteogenic Transformation of Human Mesenchymal Stem Cells

Download

index.pdf

Date

2022-07-01

Author

Kankilic, Berna
Bayramlı, Erdal
KORKUSUZ, PETEK
EROĞLU, HAKAN
ŞENER, BURÇİN
Mutlu, Pelin
KORKUSUZ, FEZA

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

99
views

39
downloads

Aims: Chronic osteomyelitis, including implant-related prosthetic joint infection, is extremely difficult to cure. We develop vancomycin containing release systems from poly(d,l-lactide) (PDLLA) and poly(d,l-lactide-co-glycolide) (PLGA) composites with beta-tricalcium phosphate (β-TCP) to treat methicillin-resistant Staphylococcus aureus osteomyelitis. We ask whether vancomycin containing PDLLA/β-TCP and PLGA/β-TCP composites will prevent early biofilm formation, allow cell proliferation and osteogenic differentiation, and stimulate osteogenic signaling molecules in the absence of an osteogenic medium. Methods: Composites were produced and characterized with scanning electron microscopy. In vitro vancomycin release was assessed for 6 weeks. Biofilm prevention was calculated by crystal violet staining. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and osteosarcoma cell (SaOS-2) proliferation and differentiation were assessed with water soluble tetrazolium salt and alkaline phosphatase (ALP) staining. Real-time quantitative polymerase chain reaction defined osteogenic signaling molecules for hBM-MSCs. Results: Totally, 3.1 ± 0.2 mg and 3.4 ± 0.4 mg vancomycin released from PDLLA/β-TCP and the PLGA/β-TCP composites, respectively, and inhibited early biofilm formation. hBM-MSCs and SaOS-2 cells proliferated on the composites and stimulated ALP activity of cells. Runt-related transcription factor 2 (RUNX2) and SRY-Box transcription Factor 9 (SOX9) expressions were, however, lower with composites when compared with control. Conclusion: Vancomycin containing PDLLA/β-TCP and PLGA/β-TCP composites inhibited early biofilm formation and proliferated and differentiated hBM-MSCs and SaOS-2 cells, but osteogenesis-related RUNX2 and SOX9 transcription factors were not strongly expressed in the absence of an osteogenic medium for 14 days.

Subject Keywords

biofilm, bone signaling molecules, PDLLA, PLGA, vancomycin, β-TCP

URI

https://hdl.handle.net/11511/98541

Journal

Frontiers in Surgery

DOI

https://doi.org/10.3389/fsurg.2022.885241

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Vancomycin containing PLLA/beta-TCP controls experimental osteomyelitis in vivo Kankilic, Berna; BİLGİÇ, ELİF; KORKUSUZ, PETEK; Korkusuz, Feza (2014-11-19) Background: Implant-related osteomyelitis (IRO) is recently controlled with local antibiotic delivery systems to overcome conventional therapy disadvantages. In vivo evaluation of such systems is however too little.
Biomechanical and histological outcome of combined raloxifene-estrogen therapy on skeletal and reproductive tissues Tasci, Arzu; BİLGİLİ, HASAN; Altunay, Hikmet; Gecit, Mehmet Rusen; Keskin, Dilek (Elsevier BV, 2010-02-10) Estrogen replacement is a potent therapy for postmenopausal osteoporosis. However, its carcinogenic effects on breasts and the uterus limit its utilization. Raloxifene has estrogen-like effects on bones without the carcinogenic symptoms on breast or uterine tissue. Their individual effects are well characterized, but the results of their interaction remains elusive. In this work, we investigate the consequences of a combined raloxifene/estrogen therapy on bone and uterus with experimental osteoporosis. 40 W...
Vancomycin release from poly(D,L-lactide) and poly(lactide-co-glycolide) disks Ozalp, Y; Ozdemir, N; Hasırcı, Vasıf Nejat (2002-01-01) A biodegradable and biocompatible polymeric system was developed for the controlled release of vancomycin for the treatment of brain abscesses. Poly(D,L-lactic acid) (PLA) and its copolymers poly( lactide-co-glycolide) PLGA 90: 10 and PLGA 70: 30, were prepared. Polymer disks containing vancomycin (VN) were prepared by solvent casting from methylene chloride solutions. Degradation of the polymer disk was studied by scanning electron microscopy, NMR and GPC. SEM revealed an increasing degree of degradation w...
Etiology of senile osteoporosis - A hypothesis Atik, O. Sahap; Uslu, M. Murad; Eksioglu, Fatih; Satana, Tolgay (Ovid Technologies (Wolters Kluwer Health), 2006-02-01) Osteoporosis is a major health problem characterized by compromised bone strength predisposing patients to an increased risk of fracture. It may cause morbidity and mortality in elderly men and women. The etiologic factors that lead to senile osteoporosis still are unclear.
Simvastatin loaded porous hydroxyapatite based microcarriers for bone tissue engineering / Güldiken, Merve; Tezcaner, Ayşen; Durucan, Caner; Department of Biotechnology (2014) Bone tissue engineering provides a new medical therapy as an alternative to conventional bone replacement grafts. Carriers designed for bone tissue engineering applications should be biocompatible, bioactive, and porous and should also meet certain minimal requirements to obtain functional engineered tissues. Polymers, ceramic materials and their composites are widely used for developing such carriers. The objective of this study was to develop and characterize a simvastatin (SIM) loaded porous hydroxyapati...

Citation Formats

B. Kankilic et al., “Vancomycin Containing PDLLA and PLGA/β-TCP Inhibit Biofilm Formation but Do Not Stimulate Osteogenic Transformation of Human Mesenchymal Stem Cells,” Frontiers in Surgery, vol. 9, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/98541.