Simvastatin loaded porous hydroxyapatite based microcarriers for bone tissue engineering /

Güldiken, Merve
Bone tissue engineering provides a new medical therapy as an alternative to conventional bone replacement grafts. Carriers designed for bone tissue engineering applications should be biocompatible, bioactive, and porous and should also meet certain minimal requirements to obtain functional engineered tissues. Polymers, ceramic materials and their composites are widely used for developing such carriers. The objective of this study was to develop and characterize a simvastatin (SIM) loaded porous hydroxyapatite microcarrier system by water in oil emulsion method for bone tissue engineering applications. In order to obtain spherical and structurally stable microcarriers, powder size should be submicron with a narrow size range. For this purpose, firstly, HAp powders (~10-17 µm) were synthesized using co-precipitation and sol-gel methods. It was found that powder characteristics synthesized by both methods were not suitable to form porous microcarriers. Therefore, the microcarriers were then prepared using Nano-HAp powders (<200nm). Spherical shaped and structurally stable microcarriers were obtained with Nano-HAp powders. The average size of microcarriers prepared by Nano-HAp was determined to be 426 µm. The fabricated microcarriers were loaded with SIM and also coated with human decellularized adipose tissue (DAT). DAT coated and SIM loaded microcarriers were used for enhancing attachment, proliferation and osteoblastic differentiation of cells as well as for controlling SIM release. Drug loadings results were 59%, 61% and 32%, for different concentrations of SIM (1, 0.5 and 0.1 mg/ml), respectively. Release kinetics of drug loaded microcarriers could not be determined because of poor water solubility of SIM (13 µg/ml in dH2O). In vitro cell viability of SIM loaded and DAT coated microcarriers were conducted by using PrestoBlue assay with two different cell types, Saos-2 and human adipose derived stem cells (hASCs) for 10 days. The results showed that although the loaded SIM amounts were higher than the toxic dose, microcarriers were not cytotoxic on both cell types. A higher cell attachment on DAT coated microcarriers was observed compared to un-coated microcarriers. A time dependent increase in cell number was observed on both coated and DAT un-coated microcarriers. Therefore, it can be concluded that both DAT coated and uncoated SIM loaded HA microcarriers have a potential in treating bone defects with tissue engineering applications.


Wet spun PCL scaffolds for tissue engineering
Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Polymeric scaffolds for bioactive agent delivery in bone tissue engineering
Uçar, Şeniz; Hasırcı, Nesrin; Yılgör, Pınar; Department of Chemistry (2012)
Tissue engineering is a multidisciplinary field that is rapidly emerging as a promising new approach in the restoration and reconstruction of tissues. In this approach, three dimensional (3D) scaffolds are of great importance. Scaffolds function both as supports for cell growth and depot for sustained release of required active agents (e.g. enzymes, genes, antibiotics, growth factors). Scaffolds should possess certain properties in accordance with usage conditions. Wet-spinning is a simple technique that ha...
Kemik Rejenerasyonunda Hızlı Prototip Teknolojisi
Kızıltay, Aysel (2015-01-01)
Kemik doku mühendisliği hasarlı kemiğin yenilenmesini amaçlar. Geleneksel üretim teknikleriyle geliştirilen iskele yapılar gözenek morfolojisinin ve yapısının kontrol edilememesi ve uyarlılığın olmaması gibi bazı kısıtlamalara sahiptir. Son yıllarda bilgisayar destekli hızlı prototip (RP) üretim teknolojisi malzeme biliminin de gelişmesiyle kemik doku mühendisliği alanında popüler hale gelmiştir. RP teknolojisini kullanan teknikler, kemik rejenerasyonu için kritik olan kontrol edilebilir ve birbirine bağlı ...
Development of microcarrier systems for bone tissue engineering
Aydoğdu, Hazal; Tezcaner, Ayşen; Baran, Erkan Türker; Department of Biomedical Engineering (2015)
Current strategies in bone tissue engineering have largely focused on development of carrier systems for repair and regeneration of bone tissue defects. The microcarrier systems offer an efficient method of delivery of cells with non-invasive injectable system. In this study, three-dimensional hydrogel microspheres were developed via water-in-oil emulsion method. In the first part of the thesis, porous pullulan (PULL) microspheres, with average size of 153±46 µm, were prepared and the surface of the microsp...
Gene Therapy Strategies in Bone Tissue Engineering and Current Clinical Applications
Atasoy-Zeybek, Aysegul; KÖSE, GAMZE (2018-01-01)
Gene therapy provides a promising approach for regeneration and repair of injured bone. Application of gene therapy has displayed increased efficiency in various animal models and preclinical trials in comparison with traditional bone grafting methods. The objective of this review is to highlight fundamental principles of gene therapy strategies in bone tissue engineering and solutions of their current limitations for the healing of bone injury. Vector types are debated for the repair of defected site due t...
Citation Formats
M. Güldiken, “Simvastatin loaded porous hydroxyapatite based microcarriers for bone tissue engineering /,” M.S. - Master of Science, Middle East Technical University, 2014.