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Versatile-in-All-Trades: Multifunctional Boron-Doped Calcium-Deficient Hydroxyapatite Directs Immunomodulation and Regeneration
Date
2022-07-11
Author
Pazarçeviren, Ahmet Engin
Akbaba, Sema
Evis, Zafer
Tezcaner, Ayşen
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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© 2022 American Chemical Society. All rights reserved.Osseointegration of implants depends on several intertwined factors: osteogenesis, angiogenesis, and immunomodulation. Lately, novel reinforcements allowing faster bonding with osseous tissue have been explored intensively. In this study, we hypothesized the use of boron as a major multifunctional ion to confer versatility to calcium-deficient hydroxyapatite (cHA) synthesized by a wet precipitation/microwave reflux method. By synthesis of boron-doped calcium-deficient hydroxyapatite (BcHA), we expected to obtain an osteoimmunomodulatory and regenerative nanoreinforcement. BcHA was found to possess a pure HA phase, a greater surface area (66.41 m2/g, p = 0.028), and cumulative concentrations of Ca (207.87 ± 6.90 mg/mL, p < 0.001) and B (112.70 ± 11.79 mg/mL, p < 0.001) released in comparison to cHA. Osteogenic potential of BcHA was analyzed using human fetal osteoblasts. BcHA resulted in a drastic increase in the ALP activity (1.11 ± 0.11 mmol/gDNA·min, p < 0.001), biomineralization rate, and osteogenic gene expressions compared to cHA. BcHA angiogenic potential was investigated using human umbilical cord vein endothelial cells. Significantly, the highest VEGF-A release (1111.14 ± 87.82 in 4 h, p = 0.009) and angiogenic gene expressions were obtained for BcHA-treated samples. These samples were also observed to induce a more prominent and highly branched tube network. Finally, inflammatory and inflammasome responses toward BcHA were elucidated using human monocyte-derived macrophages differentiated from THP-1s. BcHA exhibited lower CAS-1 release (50.18 ± 5.52 μg/gDNAμg/gDNA) and higher IL-10 release (126.97 ± 15.05 μg/gDNA) than cHA. In addition, BcHA treatment led to increased expression of regenerative genes such as VEGF-A, RANKL, and BMP-2. In vitro results demonstrated that BcHA has tremendous osteogenic, angiogenic, and immunomodulatory potential to be employed as a "versatile-in-all-trades" modality in various bone tissue engineering applications.
Subject Keywords
boron
,
hydroxyapatite/tricalcium phosphate
,
immunomodulation
,
angiogenesis
,
osteogenesis
,
PHYSICOCHEMICAL PROPERTIES
,
DIFFERENTIATION
,
BIOCOMPATIBILITY
,
VASCULARIZATION
,
ANTIBACTERIAL
,
ANGIOGENESIS
,
OSTEOGENESIS
,
MACROPHAGES
,
MIGRATION
,
STRONTIUM
,
angiogenesis
,
boron
,
hydroxyapatite/tricalcium phosphate
,
immunomodulation
,
osteogenesis
URI
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85133973936&origin=inward
https://hdl.handle.net/11511/99058
Journal
ACS Biomaterials Science and Engineering
DOI
https://doi.org/10.1021/acsbiomaterials.2c00242
Collections
Department of Engineering Sciences, Article
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A. E. Pazarçeviren, S. Akbaba, Z. Evis, and A. Tezcaner, “Versatile-in-All-Trades: Multifunctional Boron-Doped Calcium-Deficient Hydroxyapatite Directs Immunomodulation and Regeneration,”
ACS Biomaterials Science and Engineering
, vol. 8, no. 7, pp. 3038–3053, 2022, Accessed: 00, 2022. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85133973936&origin=inward.