Synthesis of peptide-fullerenol conjugate and peptide hydrogels for drug delivery

Demirci, Hande
In past decades, peptides were examined thoroughly and their use for biomedical applications enhanced largely. Peptides can be used in different forms such as selfassembly hydrogels, scaffolds, nanotubes, etc. These forms benefit various applications such as developing drugs, targeted drug delivery, wound dressings, and tissue engineering. Peptide hydrogels are used mostly for these applications that were mentioned previously. In addition, peptides can have antimicrobial properties depending on their sequence. Antimicrobial peptides (AMP) have a wide range of use in health care, animal husbandry, agriculture, aquaculture, etc. In this thesis, Ac-(FKFE)2, Ac-(FKFE)3, and Ac-IKYLSVN-NH2 peptides were synthesized based on the literature by using the Solid Phase Peptide Synthesis method. Then, gel formation of the peptides was examined in different solvents and solutions such as water, phosphate buffer saline (PBS), NaCl solution, and cell medium. Successful gel formation was observed for the peptide Ac-(FKFE)3, and Ac-IKYLSVN-NH2. It has been stated in the literature that Ac-IKYLSVN-NH2 has an antimicrobial effect. Thus, biological studies were only conducted for Ac- IKYLSVN-NH2 to examine antimicrobial susceptibility and drug release capability of the peptide. Fullerenols are water-soluble fullerene derivatives which can be functionalized by amino acids and peptides to achieve desired properties. A fullerenol-peptide conjugate was tried to be synthesized and characterized using the peptide Ac- IKYLSVN-NH2.


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Citation Formats
H. Demirci, “Synthesis of peptide-fullerenol conjugate and peptide hydrogels for drug delivery,” M.S. - Master of Science, Middle East Technical University, 2022.