Comparison of common carbon sources with unhydrolyzed, dilute acid and steam hydrolyzed distillers’ dried grains with solubles for lignocellulolytic enzyme productions by fungal strains

Date

2023-05-15

Author

Iram, Attia
Çekmecelioğlu, Deniz
Demirci, Ali

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Distillers’ dried grains with solubles (DDGS) are the byproduct of first-generation bioethanol production. DDGS has a high fiber content, which can be used for hydrolytic enzyme production after pretreatment by physical or chemical methods such as dilute acid or semi-continuous steam explosion. This study compares the treated DDGS samples to common carbon sources such as glucose, Avicel (crystalline cellulose), and untreated DDGS along with other cellulosic materials during the production of cellulases and xylanases by selected four fungal strains. Acid treated DDGS without filtration produced up to 0.72 IU/ml of cellulases and 36.44 IU/ml of xylanases. The results showed that acid hydrolysis was a better treatment than untreated, and steam treated DDGS for both cellulase and xylanase productions (p < 0.05). The comparison of enzyme productions with acid hydrolyzed DDGS to common carbon sources also showed that acid hydrolyzed DDGS was a significantly better carbon source than others. Among four fungal strains, two Aspergillus niger strains (NRRL 567 and NRRL 330) were better than the others in enzyme activity levels. The results confirm the significant positive effect of dilute acid treatment in making DDGS a feasible feedstock for hydrolytic enzyme production.

Subject Keywords

Cellulases, DDGS, Distillers’ dried grains with solubles, Xylanases

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85147294747&origin=inward
https://hdl.handle.net/11511/102200

Journal

Fuel

DOI

https://doi.org/10.1016/j.fuel.2023.127572

Collections

Department of Food Engineering, Article

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Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (Elsevier BV, 2019-06-01) Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreat...
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Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-10-01) © 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimizat...
Bacterial and fungal strain selections for cellulase and xylanase production using distillers' dried grains with solubles (DDGS) Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2020-01-01) Distillers' dried grains with solubles (DDGS) are by-products of dry-mill bioethanol production from corn and wheat. The DDGS is leftover by the yeast after converting the starch into ethanol and it has a high nutritional profile including undigested fiber and protein fractions. The DDGS can be used as cheap microbial feedstock for the production of cellulase and xylanases, which are produced from expensive industrial feedstocks. In this study, screening of several bacterial and fungal strains was done usin...

Citation Formats

A. Iram, D. Çekmecelioğlu, and A. Demirci, “Comparison of common carbon sources with unhydrolyzed, dilute acid and steam hydrolyzed distillers’ dried grains with solubles for lignocellulolytic enzyme productions by fungal strains,” Fuel, vol. 340, pp. 0–0, 2023, Accessed: 00, 2023. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85147294747&origin=inward.