Preparation of Barium doped Baghdadite/PHBV fibrous scaffolds for bone tissue engineering

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2023-1-23

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Sadreddini, Sanaossadat

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Recently, bioceramic/polymer composites have dragged a lot of attention for treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop fibrous composite poly(hydroxybutyrate) co (hydroxyvalerate)- polycaprolactone, PHBV-PCL, scaffolds containing different amounts of baghdadite (BAG) and Ba-doped BAG that can provide bone regeneration in the bone defect area and to investigate the effect of these scaffolds on the structural, mechanical, and biological properties. BAG and Ba-doped BAGs were synthesized using the sol-gel method and sintered at 1150oC. Microstructural and biological characterization results were evaluated and 4 different groups were selected for scaffold constructions. PHBV/PCL composite scaffolds containing different amounts of BAG and Ba-doped BAG (1, 3, and 5 wt%) were produced by the wet electrospinning method with a porosity of 72-78%. The presence of Ba-doped BAG in the PHBV scaffolds resulted in increasing bioactivity, cell viability, and ALP activity, and it was introduced as a suitable way to control the degradation rate of scaffolds. The presence of BAG in the scaffolds improved the compressive strength of the scaffolds. The compressive strength of the scaffolds was between 4.69-9.28 vi kPa and the 5% Ba0.3-BAG+PHBV/PCL scaffold was found to have the maximum compressive strength. In the relative cell viability (%) test, the highest viability was observed on the scaffolds with BAG. The viability changes were very small between groups and contradictory with Ba content for different BAG % groups. It was concluded that PHBV/PCL electrospun scaffold with 5% Ba-BAG has the potential to be used in BTE.

Subject Keywords

Ba doped baghdadite,, PHBV/PCL, Composite scaffolds, Bone tissue engineering, Electrospinning

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https://hdl.handle.net/11511/102044

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

S. Sadreddini, “Preparation of Barium doped Baghdadite/PHBV fibrous scaffolds for bone tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2023.