Studies on the development of osmotic stress resistant wheat (Triticum aestivum L.) by introducing aldose reductase gene through particle bombardment

Ertuğrul Setenci, Fahriye
Environmental stresses come in many forms; the most prevalent stresses commonly exert their effects by changing the plant water status. In response to the decrease in water potential caused by drought, low temperature, or high salinity, certain osmoprotectant compounds such as proline, other amino acids and polyamines and hydroxy compounds (sucrose, polyols and oligosaccharides) are accumulated in vascular and nonvascular plants. Pathways involving these compounds may be exploited to produce stress- tolerant plants by genetic engineering techniques. Aldose reductase belongs to the aldo-keto reductase superfamily, monomeric NADPH-dependent oxidoreductase with broad substrate specificity, ranging from aldose sugar to aromatic aldehydes. It catalyses the first step of the polyol reaction route; the reduction of D-glucose to sorbitol. It also works as 111detoxificant for the reduction of toxic aldehydes, generated from the reactions of free radicals and membrane lipids, by converting them into alcohols. Aldose reductase gene, ALR, isolated from alfalfa (MsALR), was constructed into a plant expression vector, and then named as pAHALR. Wheat immature embryos and suspension cultures were bombarded with this plasmid and transformed immature embryos were cultured on medium containing 10 mg/L Bialaphos or PPT as selective agent. For the molecular analysis of putative transgenic samples, PCR, Southern and Western blots were carried out. Also aldose reductase enzyme activity was shown by HPLC only in transgenic samples by the accumulation of sorbitol, which is the end product of aldose reductase catalyzed glucose reduction reaction. Protective function of ALR was verified by MDA analysis under stress conditions. Regeneration of transformed calli, which were proven by molecular analysis, is brought to a certain point and still in progress. In the future, we are planning to complete regeneration studies and obtain fully fertile plants. The activity of the gene will also be investigated on these plants by biochemical and physiological analysis.


Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae
Uyar, Ebru Ozer; Yucel, Meral; Hamamcı, Haluk (2016-05-01)
Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8kDa. The amino acid sequence of RoTPS1 has a relatively high homology with th...
An investigation on value orientations, attitudes and concern towards the environment: the case of Turkish elementary school students
Onur, Arzu; Şahin, Elvan; Öztekin, Ceren (2012-01-01)
Environmental attitudes depend on the relative importance that individuals attach to themselves, other people, or all living things. These distinct bases have been found to predict environmental concern, and may act as statistically significant determinants of pro-environmental behaviours. We claim that examining the complex nature of value orientations and concern levels among elementary school students as well as the interrelationships between these attributes could guide researchers and educators in Turk...
A Study on turkish elementary school students’ nature relatedness, environmentally responsible behaviors and motive concerns
Bahar, Fatma; Şahin, Elvan; Department of Elementary Science and Mathematics Education (2015)
Environmental deterioration has reached a critical point in recent years all over the world. Children with their potential in resolution of environmental problems must be equipped with the necessary skills, knowledge, values and attitudes. Thus, this study aimed to assess (1) Turkish elementary students’ (N=1774) environmentally responsible behaviors, connections with nature (nature relatedness), and motive concerns and (2) the relationship of elementary school students’ environmental responsible behaviors ...
Enhanced salt tolerance of transgenic tobacco expressing a wheat salt tolerance gene
KAVAS, MUSA; BALOĞLU, MEHMET CENGİZ; Yücel, Ayşe Meral; Öktem, Hüseyin Avni (2016-01-01)
Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants i...
Transformation of tobacco with a NAC type transcription factor, tanac69-1 and characterization of transgenic plants via molecular and physiological techniques
Eroğlu, Ayten; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biotechnology (2015)
Environmental stresses such as drought and salinity greatly affect plant production leading to reduction in yield and quality. Stress tolerant plants can be rapidly produced by transferring stress related genes or transcription factors to crop plants. In this study, TaNAC69-1 gene, a NAC type transcription factor, was isolated from Triticum aestivum L. cv. Yüreğir-89 and cloned into a Gateway compatible overexpression vector, pEarleyGate 100. Transformation of Nicotiana tabacum L. cv. Samsun leaves was carr...
Citation Formats
F. Ertuğrul Setenci, “Studies on the development of osmotic stress resistant wheat (Triticum aestivum L.) by introducing aldose reductase gene through particle bombardment,” Ph.D. - Doctoral Program, Middle East Technical University, 2002.