In vitro bone tissue engineering on patterned biodegradable polyester blends

Download

143156.pdf

Date

2003

Author

Kenar, Halime

Metadata

Show full item record

Item Usage Stats

205
views

0
downloads

This study aimed at guiding osteoblast cells on biodegradable polymer carriers with well-defined surface microtopography and chemistry, and investigating the effect of cell alignment on osteoblast phenotype expression. A blend of two different polyesters, one being natural in origin (PHBV) and the other synthetic (P(L/DL)LA), was used to form a film with parallel macro- (250 um wide) or microgrooves (27 jam wide) on its surface, by solvent casting on patterned templates. The micropatterned Si template was produced by photolithography, while the Teflon macropatterned template was lathe cut. Fibrinogen (Fb) was adsorbed or immobilized via epichlorohydrin spacer/crosslinker on the film surfaces to enhance cell attachment by increasing the surface hydrophilicity and by providing RGD amino acid sequence for integrin binding. Surface hydrophilicity was assessed by water contact angle measurements. Adsorption of Fb caused an increase in hydrophilicity, while the opposite was achieved with its covalent immobilization. Fb was homogeneously immobilized throughout the whole micropatterned film surface with amount of 153.1 + 42.4 g Fb/cm, determined with the Bradford assay, while it was adsorbed within the grooves of the micropattern. Surface characteristics of the films were studied with Scanning Electron (SEM) and Light microscopy. Osteoblast cells derived from rat bone marrow were seeded on the polymeric films with different surface topography and chemistry and were grown for one and three weeks. Osteoblast proliferation on the films was determined with Cell Titer 96 T Non-Radioactive Cell Proliferation (MTS) test. Alkaline Phosphatase (ALP) assay and tetracycline labelling of mineralized matrix were carried out to determine osteoblast phenotype expression on different surfaces. SEM and fluorescence microscopy were used to evaluate the cell alignment. Osteoblasts on the micropatterned films with adsorbed Fb aligned along the groove axis with a mean deviation angle of 13.1°, while on the unpatterned films deviation from horizontal axis was 63.2° and cells were randomly distributed. Cell alignment did not affect cell proliferation. However, the highest ALP specific activity and the most homogeneous mineral distribution were obtained on the Fb adsorbed micropatterned films.

Subject Keywords

Chemical engineering, Bone remodeling, Polyesters, Bone tissue engineering, Patterned surfaces, Biodegradable, Polymer films, Osteoblast guidance, Osteoblast phenotype expression

URI

https://hdl.handle.net/11511/13766

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

In vivo response to biodegradable controlled antibiotic release systems Korkusuz, F; Korkusuz, P; Eksioglu, F; Gursel, I; Hasırcı, Vasıf Nejat (2001-05-01) In this study, the major goal was to evaluate in vitro and in vivo findings by macroscopy, radiology, and histology to determine the effectiveness of therapy of experimental implant-related osteomyelitis with antibiotic carrier rods constructed of microbial polyesters. The polymers used were poly(3-hydroxybutyrate-co-4-hydroxyvalerate) [P(3HB-co-4-HB)] and poly(3-hydroxybutyrate-co-3-hydroxy-valerate) [P(3-HB-co-3-HV)]. Both the Sulperazone(R) and the Duocid(R)-P(3-HB-co-4-HB) rods with a drug to polymer ra...
In vitro and transdermal penetration of PHBV micro/nanoparticles Eke, G.; Kuzmina, A. M.; Goreva, A. V.; Shishatskaya, E. I.; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2014-06-01) The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cel...
In vitro Comparison of Autoclave Polymerization on the Transverse Strength of Denture Base Resins Durkan, Rukiye; ÖZEL, MEHMET BİROL; Bagis, Bora; Usanmaz, Ali (Japanese Society for Dental Materials and Devices, 2008-07-01) The aim of this study was to determine the effect of autoclave polymerization on the transverse strength of denture base polymers. To this end, 30 rectangular test specimens were fabricated of two heat-polymerized denture base polymers. The test groups were: (I) control. i.e.. conventional water bath to polymerize result by heat at 100 degrees C for 30 minutes; (II) autoclave polymerization at 60 degrees C for 30 minutes followed by 130 degrees C for 10 minutes; and (III) autoclave polymerization at 60 degr...
A DEP-Based Lab-On-A-Chip System For The Detection Of Multidrug Resistance In K562 Leukemia Cells Yalçın, Yağmur Demircan; Özkayar, Gürkan; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk (2016-06-09) This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100% selectivity at a flow rate of...
A Five Layer One-Dimensional PEMFC Model with Detailed Electrode Kinetics Ficicilar, B.; Eroğlu, İnci; Nguyen, T. V. (2010-10-01) In this study, a non-isothermal, two-phase, one-dimensional, and steady state proton exchange membrane (PEM) fuel cell model is developed to investigate the detailed electrode kinetics in the anode and cathode catalytic regions and the effects of these reaction mechanisms on the performance of a PEM fuel cell. Langmuir-Butler-Volmer (LBV) type reaction rate expressions are suggested for both anode and cathode electrochemical reactions. A two-phase energy balance approach is used to catch the thermal transpo...

Citation Formats

H. Kenar, “In vitro bone tissue engineering on patterned biodegradable polyester blends,” M.S. - Master of Science, Middle East Technical University, 2003.