Inhibitory effects of plant originated extracts on bovine lens aldose reductase

Download

index.pdf

Date

2004

Author

Zaimoğlu, Selin

Metadata

Show full item record

Item Usage Stats

218
views

105
downloads

Aldose reductase, E.C.1.1.1.21, catalyzes the reduction of different types of aldehydes to their corresponding alcohols, and especially reduces various aldo-sugars using NADPH as the coenzyme. Under hyperglycemic conditions aldose reductase is involved in the development of diabetic complications. As a result, interest has been placed over the years on the development of potent aldose reductase inhibitors for possible use in the therapy of these severe diabetic complications. In this study, aldose reductase was isolated from bovine lens by differential centrifugation and ammonium sulfate precipitation. The conditions for the enzyme assay; such as substrate (DL-Glyceraldehyde) and coenzyme (NADPH) concentration, protein amount, effect of sulfate ions, temperature and pH on the enzyme activity were optimized. The inhibitory effects of Punica granatum, Spinacia olaeracea, Allium cepa Allium porrum, Malus flouribunda, Malus domestica extracts were tested on crude bovine lens aldose reductase. Four different types of organic fractions from each crude plant extract were obtained by solvent fractionation. The inhibitory activity of these organic fractions was calculated considering the aldose reductase activity without extracts as 100 %. All six plants were found to inhibit aldose reductase activity to different extent. Among these fractions obtained as; petroleum ether, diethyl ether, ethyl acetate, and n-butanol. Highest inhibitory activity was found for the ethyl acetate fraction. The IC50 values of ethyl acetate fractions of all these plants was calculated as, 25.46 æg/ml, 20.5 æg/ml, 18.5 æg/ml, 12.32 æg/ml, 6.45 æg/ml, 5.4 æg/ml, for Allium porrum, Malus domestica, Spinacia olaeracea, Malus floribunda Allium cepa, Punica granatum respectively.

Subject Keywords

Research.

URI

http://etd.lib.metu.edu.tr/upload/2/12605076/index.pdf
https://hdl.handle.net/11511/14159

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Preparation of cross-linkedtyrosinase aggregates Aytar, Burcu Selin; Bakır, Ufuk; Department of Chemical Engineering (2006) The aim of this study was to prepare cross-linked enzyme aggregate (CLEA) from crude mushroom (Agaricus bisporus) extract. However, the optimization of CLEA production was performed by using pure tyrosinase. Important parameters were determined as protein, ammonium sulfate and glutaraldehyde concentrations, CLEA particle size, and cross-linking temperature and period. On the other hand, the order of ammonium sulfate and glutaraldehyde addition did not affect the yield of CLEA. Optimum CLEA preparation condi...
Free forming of locally laser heated parts Özmen, Murat; Tekkaya, A. Erman; Department of Mechanical Engineering (2005) As metals have high formability at elevated temperatures, hot forming is preferred and widely used in manufacturing of complicated geometries. The term أhot formingؤ is usually used if the whole workpiece is processed at elevated temperatures. However, for certain products high formability is required only locally. Forming by local heating is proposed to provide ease of manufacturing of local forms on the workpiece. Also, tools can be simplified by this method. In this study, local laser heating procedures ...
Removing algae from stabilization pond effluents by using trickling filters Kaya, Devrim; Dilek, Filiz Bengü; Department of Environmental Engineering (2005) The objective of this study is to remove turbidity originating from algae present in oxidation ponds effluents by an easy and inexpensive method. For this reason, a novel lab-scale Step Feed Dual Treatment (SFDT) process was constructed and the efficiency of trickling filter (TF) to remove algae and organic matter was investigated. SFDT process developed in this study is the unique, inexpensive and new system to scavenge algae from oxidation pond effluents. In this system, influent is first treated in a sta...
Amination/annulation of chlorobutenones with chiral amine compounds: synthesis of 1,2,4-trisubstituted pyrroles Demir, Ayhan Sıtkı; Igdir, AC; Gunay, NB (Elsevier BV, 2005-10-03) A series of 1,2,4-trisubstituted pyrroles have been synthesized in 83-96% yields on treatment of chiral primary amines, amino alcohols and esters of alpha-amino acids with different chlorobutenones in benzene-triethylamine. The conversions proceed without racemization.
Effects of the non-steroidal anti-inflammatory drug celecoxib on cholesterol containing distearoyl phosphatidylcholine membranes Sade, Asli; Banerjee, Sreeparna; Severcan, Feride (2011-01-01) The effects of different concentrations of celecoxib on the acyl chain order, dynamics and the hydration status of the head group and interfacial region of model membranes containing DSPC and cholesterol were investigated in detail using Fourier transform infrared spectroscopy. Our results reveal that regardless of the presence of cholesterol, celecoxib is able to alter the physical properties of membranes. It exerts opposing effects on membrane order at high and low concentrations and decreases membrane fl...

Citation Formats

S. Zaimoğlu, “Inhibitory effects of plant originated extracts on bovine lens aldose reductase,” M.S. - Master of Science, Middle East Technical University, 2004.