Optimisation of agrobacterium mediated gene transfer and micrografting systems in lentil (lens culinaris medik)

Kamçı, Hamdi
In this work Agrobacterium (KYRT1::pTJK136) mediated gene transfer to lentil (Lens culinaris Medik.) embriyo apex and regeneration through micro-grafting in lentil was studied. In micro-grafting two different types root stock stem height and root stock preparations were optimized. According to the results half stem length was found to be more successful then the full. Also lentil root stock was more successful then the chickpea root stock. The types of root stock preparations studied were designated as Z and M. The Z type root stock was superior then the M type, when the micro-grafting, hardening and green-house stages were concerned. In study of Agrobacterium mediated gene transfer to lentil embryo apex the effect of the following parameters on the transformation efficiency were addressed; type and intensity of injury, type of pre-incubation media for injured explants, effect of evacuation, effect of L-cysteine during co-cultivation and Agrobacterium incubation duration. According to the results crushing type of injury was superior over the poking and sonication type of injuries. Following the injury Hoglands solution was used as pre-incubation media prior to infection. The effect of evacuation parameter was found to be insignificant whereas the effect of L-cysteine during co-cultivation was negative on the transformation efficiency. According to the Agrobacterium incubation duration studies, 240 minutes, followed by 120 minutes of Agrobacterium incubation were the most efficient in terms of transformation efficiency. However, since there was no significant difference among the two 120 minutes chosen to be the optimum bacterial incubation duration.


In vitro induction of growth and development of common juniper(Juniperus communis L.) from shoot and bud explants
Koçer, Zeynep Ahsen; Kaya, Zeki; Department of Biotechnology (2005)
The objective of the study was to investigate the optimum conditions for in vitro regeneration of common juniper (Juniperus communis L.) by using indirect organogenesis approach. Throughout the study; callus induction, organogenesis, improved organogenesis and root induction experiments were performed sequentially. It was found that explant position, genotype, gender, treatments and sampling time had significant effects on callus induction rate in common juniper. The results of treatments indicated that IBA...
Neurosecretory cells in the molluses Helix have perikarya that show clear signs of adaptation for both the synthesis and the discharge of secretory material. They are characterized morphologically by juxtaposition with the neural lamella. Passage of hormone into the haemocoel is apparently facilitated in many cases by the extreme attenuation of areas of the lamella and perineurium adjacent to the perikarya and by other forms of histological differentiation. Presumptive sites of release are characterized by ...
Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with enzyme electrodes
Yildiz, HB; Kiralp, S; Toppare, Levent Kamil; Yagci, Y (Elsevier BV, 2005-12-15)
Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl methacrylate). Immobilization of enzyme was carried out by the entrapment in conducting polymers during electrochemical polymerization of pyrrole on the copolymer electrodes. Maximum reaction rate, Michaelis-Menten constants, temperature, pH and operational stabilities were determined for immobilized enzyme. The amount of glucose in orange juices of Turkey was investigated by using enzyme...
Optimization of selection conditions and agrobacterium mediated transformation of chickpea (cicer arietinum L. cv. Gökçe)
Öz, M. Tufan; Öktem, Hüseyin Avni; Department of Biotechnology (2005)
The objective of this study was to optimize an efficient selection system and Agrobacterium mediated transformation of chickpea (Cicer arietinum L.). Cotyledonary node explants of Turkish chickpea cultivar Gökçe were used to determine the effects of selective agents, two antibiotics (Kanamycin, Hygromycin) and two herbicides (PPT, Glyphosate) as well as four antibiotics (Augmentin, Carbenicillin, Cefotaxime, Timentin) for eliminating Agrobacterium on multiple shoot and root induction. Selective agents and a...
Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin.
Vasiljeva, L; Kim, M; Terzi Çizmecioğlu, Nihal; Soares, LM; Buratowski, S (Elsevier BV, 2008-02-15)
Within the heterochromatin of budding yeast, RNA polymerase II (RNAPII) transcription is repressed by the Sir2 deacetylase. Although heterochromatic silencing is generally thought to be due to limited accessibility of the underlying DNA, there are several reports of RNAPII and basal transcription factors within silenced regions. Analysis of the rDNA array revealed cryptic RNAPII transcription within the "non-transcribed" spacer region. These transcripts are terminated by the Nrd1 /Sen1 complex and degraded ...
Citation Formats
H. Kamçı, “Optimisation of agrobacterium mediated gene transfer and micrografting systems in lentil (lens culinaris medik),” M.S. - Master of Science, Middle East Technical University, 2004.