Evaluation of effectiveness of different bioactive agents for treatment of osteoarthritis with in vitro model under dynamic mechanical stimulation

Kavas, Ayşegül
Osteoarthritis (OA) is a disease characterized by the progressive degradation of articular cartilage. Current strategies for the disease are mainly towards relieving symptoms. This study was aimed to investigate the therapeutic potentials of Bone Morphogenetic Protein-9 (BMP-9), Raloxifene (Ral) and Pluronic F-68 (PLF-68) with a three-dimensional in vitro OA model. Articular chondrocytes isolated from rats were cultured in growth media and embedded in agarose to obtain agarose-chondrocyte discs. Dynamic hydrostatic mechanical stress was applied to discs. The discs were incubated with Aza-C for 48 hours for OA development. After its removal, chondrocytes were treated with different doses of BMP-9, Ral and PLF-68 for 10 days. The efficacies of treatments were evaluated by measuring cell number, glycosaminoglycan and collagen amount, and mechanical properties of the v discs. Measurements of these properties were performed with MTT, quantitative colorimetric assays, histochemical staining and mechanical tests, respectively. According to comparative results with healthy groups and controls (osteoarthritic chondrocytes without any treatment), it was found that BMP-9 had negative effect on osteoarthritic chondrocytes. On the other hand, Ral showed positive results related with matrix synthesis and mechanical properties especially at 5 μM dose suggesting that it holds promise for the treatment of OA. The therapeutic effect of Ral on OA was documented for the first time in literature. The potential of PLF-68 for treatment of OA was also supported by this study considering its positive effects on cell number, collagen synthesis and mechanical properties. Yet, further investigations are also suggested for conclusive results on this agent.


Multiwalled Carbon Nanotube- Poly(2-Hydroxyethyl Methacrylate) Composite Conduitfor Peripheral Nerve Repair
Arslantunalı, Damla; Arslantunalı Şahin, Damla; Department of Biotechnology (2012)
There are different methods used in the surgical treatment of peripheral nerve injury. In this respect, end-to-end surgical reconnection of the damaged nerve ends or autologous nerve grafts are applied as soon as possible after the injury. When autologous tissue transplant is considered, there are some medical devices available generally for relatively short nerve defects. As a solution for this problem, different tissue engineered nerve conduits have been developed. In the current study, a pHEMA hydrogel m...
Development and characterization of injectable alginate/sericin/chondroitin sulphate microspheres for cartilage tissue engineering /
Göl, Nil; Keskin, Dilek; Korkusuz, Feza; Department of Biotechnology (2015)
Osteoarthritis (OA) is one of the joint diseases with the highest prevalence worldwide. Eighty percent of the OA patients present limitation in joint movements and 25% cannot perform their daily activities. Due to loss of regenerative potency in OA joints, treatments with drugs and bioactive agents can provide only limited recovery in the damaged cartilage tissue. Current studies and clinical approaches therefore, mostly focus on cell based treatments with hydrogel carriers. The microcarrier systems supply ...
Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis
Koc, Dilara; Begentaş, Onur Can; Yurtogullari, Sukran; Temel, Musa; Akcali, Kamil Can; Demirkaya, Seref; Kiriş, Erkan (2021-05-01)
Multiple sclerosis is a chronic disease characterized by inflammation, demyelination, and axonal damage in the central nervous system. Here, we established an induced pluripotent stem cell (iPSC) line METUi001-A from the peripheral blood mononuclear cells of a 25-year-old male individual with clinically diagnosed Relapsing-Remitting Multiple Sclerosis (RRMS) using the integration-free Sendai reprogramming method. We demonstrated that the iPSCs are free of exogenous Sendai reprogramming vectors, have a norma...
The effect of indole acetic acid, abscisic acid, gibberellin and kinetin on the expression of arf1 gtp binding protein of pea (pisum sativum l. cv. araka)
Ertekin, Özlem; Memon, Abdulrezzak; Department of Biology (2007)
ADP Ribosylation Factor 1 (ARF1) is a universal small GTP binding protein which has an important role in vesicular trafficking between endoplasmic reticulum and Golgi. ARF1 is a basic component of Coat Protein I (COPI) vesicles which have functions in both formation of coatomer complex and recruitment of cargo proteins. In this study, the expression ARF1 was analyzed in pea (P. sativum L. cv. Araka) grown at different developmental stages. Because of the differential hormonal levels at corresponding stages,...
Elmazoglu, Z.; Colakoglu, M.; Banerjee, Sreeparna; Bitik, B.; Aktekin, C. N.; Goker, B.; Karasu, C. (2018-06-01)
Background Osteoarthritis (OA), is a leading cause of joint dysfunction and the disease is characterised by progressive destruction of the articular cartilage.¹ At the molecular level, degeneration of the cartilage is attributed to multifactorial events including oxidative stress, mitochondrial dysfunction, apoptosis and associated changes in inflammatory and catabolic gene expression.² Recent studies have revealed the essential role of plant-derived antioxidants in preventing pathophysiological events obse...
Citation Formats
A. Kavas, “Evaluation of effectiveness of different bioactive agents for treatment of osteoarthritis with in vitro model under dynamic mechanical stimulation,” M.S. - Master of Science, Middle East Technical University, 2007.