Effect of cold stress on antioxidant mechanism of winter and spring type barley (hordeum vulgare L.) cultivars

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2007

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Afşar, Nilüfer

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In this study, effect of cold stress on physiology and biochemistry of two Turkish barley cultivars, winter type Tarm-92 and spring type Zafer-160, was studied. For chilling stress treatment, cultivars were exposed to +4 ºC for 1, 3 and 7 days, and for freezing stress application acclimated cultivars (+4 ºC for 3 days) were treated with -3 ºC and -7 ºC. After freezing stress treatment, a recovery period was applied for 4 days at 4 ºC. Following analyses were performed on leaf and root tissues: growth parameters (length, wet-dry weights), malondialdehyde (MDA) content, proline content, hydrogen peroxide content (H2O2) electrolyte leakage, PS II fluorescence (Fv/Fm), antioxidant enzyme activities such as catalase (CAT: EC 1.11.1.6), ascorbate peroxidase (APX: EC 1.11.1.11) and glutathione reductase (GR: EC 1.6.4.2). It was observed that effect of cold was more at freezing temperatures than chilling temperature. Cold dependent damage was more obvious as the duration of chilling temperature increased. Growth retardation, membrane damage, leaf catalase deactivation were more apparent and leaf glutathione reductase activity increase was less in spring type cultivar Zafer than in winter type Tarm. These results indicated that winter type barley cultivar is more cold tolerant than spring type barley.

Subject Keywords

Biochemistry.

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http://etd.lib.metu.edu.tr/upload/3/12608514/index.pdf
https://hdl.handle.net/11511/16895

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

N. Afşar, “Effect of cold stress on antioxidant mechanism of winter and spring type barley (hordeum vulgare L.) cultivars,” M.S. - Master of Science, Middle East Technical University, 2007.