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Determination and comparison of genetic variation in honey bee (Apis mellifera L.) populations of Turkey by Random Amplified Polymorphic DNA and microsatellite analyses
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Date
2009
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Ivgin Tunca, Rahşan
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We analyzed a total of 760 worker bees, two samples per colony, 390 colonies in 26 provinces in Turkey to determine and compare the genetic variation of Turkish honey bee (Apis mellifera L.) populations using 10 primers for RAPD and 6 microsatellite loci. Mean gene diversity levels ranged from 0.035 (Sanlıurfa) to 0.175 (Antalya) for RAPD and 0.449 (Muğla) to 0.739 (Artvin) for microsatellite markers. Private band patterns and alleles, pairwise FST values support that the Anatolian honey bees belong to C lineage except for Hatay and Sanlıurfa populations illustrated from previous findings of mitochondrial DNA studies. Genetic differentiation (GST) from RAPD data ranged from 0.060 (Bilecik and Muğla) to 0.395 (Gökçeada and Sanlıurfa). The genetic diversity (FST) for microsatellites ranged from -0.068 (Gökçeada and Đzmir) to 0.347 (Konya and Muğla). The results of the present research are in agreement to that of previous study in Turkish honey bee populations which used different microsatellite loci. That is the genetic variation was the highest in African, the lowest in European and intermediate in the Mediterranean honey bee populations. The data presented here indicate that in spite of extensive migratory beekeeping, there is still a large genetic differentiation among honey bee populations. These results should be considered in establishment of conservation plans particularly in moving of colonies between regions. The most importantly introduction of bees with foreign origin and distribution queen bees from one center to all over the country which will homogenize the gene pool of the populations should be prevented
Subject Keywords
Biology.
,
Genetics.
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http://etd.lib.metu.edu.tr/upload/3/12610443/index.pdf
https://hdl.handle.net/11511/18512
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Graduate School of Natural and Applied Sciences, Thesis
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R. Ivgin Tunca, “Determination and comparison of genetic variation in honey bee (Apis mellifera L.) populations of Turkey by Random Amplified Polymorphic DNA and microsatellite analyses,” Ph.D. - Doctoral Program, Middle East Technical University, 2009.