Morphometric, mtdna and microsatellite analysis in honeybee populations (apis mellifera L.) of north and northwest İran

Farhoud, Houman Jabbari
Morphometric measurements, mitochondrial DNA analyses and 5 microsatellite loci were used to investigate variation in the honeybee populations of Iran and comparing it with the Turkish populations. Five honeybee populations were sampled from North and west north of Iran. In morphometric aspect of the study 23 characters were measured from left forewings and hindlegs of honey bee samples. The data were analysed by multivariate statistical analyses. By using mtDNA analyses length polymorphism of the intergenic region COI-COII of mitochondrial DNA was studied. After amplification of this region by the polymerase chain reaction, DraI enzyme was used for restriction of amplified region. Results of mtDNA studies show no diversity between four populations and all of them exhibit the same C1 pattern. Five microsatellite loci (A7, A24, A28, A43 and A113) were used in this studies.A high level of average heterozygosity changing between 0.611 and 0.709 was detected in Iranian honey bee populations, and a significant degree of polymorphism was observed. Although Urmia, Sarein and Viladereg populations are similar, Amol population which has located in northern Iran shows a significant difference from other populations. Result obtained form morphometric studies are supporting microsatellite analyses. By comparing data obtained form Iranian honey bee populations with Turkish population (Hakkari), western populations (Urmia, Sarein and Viladereg) are more similar to Hakkari population. It is found Amol is significantly different form other populations and better represents Iranian honeybee.
Citation Formats
H. J. Farhoud, “Morphometric, mtdna and microsatellite analysis in honeybee populations (apis mellifera L.) of north and northwest İran,” M.S. - Master of Science, Middle East Technical University, 2004.