Scale up of panel photobioreactors for hydrogen production by PNS Bacteria

Avcıoğlu, S. Gökçe
Production of hydrogen from biomass through the use of dark and photofermentative bacteria will be applicable in the future and a promising route. The aim of this study is to develop and to scale-up solar panel photobioreactors for the biological hydrogen production by photosynthetic purple non sulfur (PNS) bacteria on artificial substrates and on real dark fermentation effluent of molasses. The parameters studied are light intensity, temperature, feed stock, feed rate, pH, cell density, light and dark cycle and carbon to nitrogen ratio on hydrogen production. Continuous hydrogen production has been achieved on artificial medium and dark fermentor effluent of molasses containing acetate and lactate by Rhodobacter capsulatus wild type and (hup-) mutant strains in panel photobioreactors in indoor and outdoor conditions by fed batch operation. Laboratory (from 4 to 8 liters) and large scale (20 L) panel photobioreactors by using various designs and construction materials were developed. In this photobioreactors continuous hydrogen production was achieved by feeding. Na2CO3 can be used as buffer to keep the pH stable during long term operation on molasses dark fermentor effluent. The adjustment of the feedstock by dilution and buffer addition were found to be essential for the long term stability of pH, biomass and H2 production for both in indoor and outdoor applications.


Demonstration and optimization of sequential microaerobic dark- and photo-fermentation biohydrogen production by immobilized Rhodobacter capsulatus JP91
Sağır, Emrah; Yucel, Meral; Hallenbeck, Patrick C. (Elsevier BV, 2018-02-01)
Hydrogen generation from complex substrates composed of simple sugars has the potential to mitigate future worldwide energy demand. The biohydrogen potential of a sequential microaerobic dark- and photo-fermentative system was investigated using immobilized Rhodobacter capsulatus JP91. Biological hydrogen production from glucose was carried out using a batch process and a bench-scale bioreactor. Response surface methodology with a Box-Behnken design was employed to optimize key parameters such as inoculum c...
Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review
Ozturk, Sibel; Çalık, Pınar; Ozdamar, Tuncer H. (Elsevier BV, 2016-04-01)
Bacillus subtilis is a highly promising production system for various biomolecules. This review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the approaches to design the initial production medium and/or feed stream. Additionally, the feeding strategies developed with or without feedback control for fed-batch B. subtilis fermentations were compiled with a special emphasis on recombinant protein (r-protein) production. For biomolecule production by wild-type B. subtilis, due t...
Obtaining durable enzyme powder via spray drying
Namaldı, Ayşegül; Adalı, Orhan; Department of Chemical Engineering (2005)
Serine alkaline protease (SAP, EC produced by Bacillus species, that are the microbioreactors within the bioreactors, is one of the major industrial enzymes. In this study, after production by Recombinant Bacillus subtilis (BGSC-1A751), carrying pHV1431::subc gene in the complex media and separation of solids, SAP was dried by using a spray drier. Experiments were performed to investigate the stabilization of SAP during spray drying and subsequent storage. Initially, the effect of air inlet tempe...
Biological hydrogen production by using co-cultures of PNS bacteria
Baysal, Görkem; Yücel, Ayşe Meral; Özgür, Ebru; Department of Biotechnology (2012)
Biological hydrogen production is a renewable, carbon-neutral and clean route for hydrogen production. Purple non-sulfur (PNS) bacteria have the ability to produce biohydrogen via photofermentation process. The type of the bacterial strain used in photofermentation is known to have an important effect on hydrogen yield. In this study, the effect of different co-cultures of PNS bacteria on photofermentation process was investigated in search of improving the hydrogen yield. For this purpose, growth, hydrogen...
Investigation Of Influencing Factors For Biological Hydrogen Production By R. Capsulatus In Tubular Photo-Bioreactors
Boran, E.; Ozgur, E.; Gebicki, J.; van der Burg, J.; YÜCEL, MUSTAFA; Gündüz, Ufuk; Modigel, M.; Eroglu, I. (2009-05-13)
Biological hydrogen production processes are considered as an environmentally friendly way to produce hydrogen. They offer the chance to produce hydrogen from renewable energy sources, like sunlight and biomass. This study aims the process development for a photo-fermentative hydrogen production by photosynthetic purple-non-sulfur bacteria, Rhodobacter capsulatus, in a large scale (80L) tubular photo-bioreactor, in outdoor conditions, using acetate as carbon source. It was shown that Rhodobacter capsulatus ...
Citation Formats
S. G. Avcıoğlu, “Scale up of panel photobioreactors for hydrogen production by PNS Bacteria,” M.S. - Master of Science, Middle East Technical University, 2010.