Preperation and characterization of silica coated magnetite nanoparticles and labeling with nonradioactive Re as a surrogate of Tc-99m for magneticly targeted imaging

Download

index.pdf

Date

2010

Author

Zengin, Ümit

Metadata

Show full item record

Item Usage Stats

183
views

69
downloads

Magnetic nanoparticles have been used in many areas owing to their variable characteristic behaviors. Among these iron oxide nanoparticles are one of the mostly preferred type of nanoparticles. In this study Fe3O4, namely magnetite, which is one type of magnetic iron oxide nanoparticles was used. Magnetite nanoparticles with a narrow size distribution were prepared in aqueous solution using the controlled coprecipitation method. They were characterized by electron microscopic methods (SEM and TEM), crystal structure analysis (XRD), particle size analyzer, vibrating sample magnetometer (VSM) and Raman spectrometry. The nanoparticles were coated with a thin (ca 20 nm) silica shell utilizing the hydrolysis and the polycondensation of tetraethoxysilane (TEOS) under alkaline conditions in ethanol. The presence of silica coating was investigated by energy dispersive X-ray spectrometer (EDX) measurement. After surface modification with an amino silane coupling agent, (3-Aminopropyl)triethoxysilane, histidine was covalently linked to the amine group using glutaraldehyde as cross-linker. Carbonyl complexes of rhenium [Re(CO)3(H2O)3]+ was prepared through reductive carboxylation utilyzing gaseous carbon monoxide as a source of carbonyl and amine borane (BH3NH3) as the reducing agent. The complex formation was followed by HPLC- ICP-MS system and 95% conversion of perrhanete into the complex was achieved. The magnetic nanoparticles were then labeled with the Re complex with a yield of 86.8% through the replacement of labile H2O groups with imidazolyl groups. Thus prepared particles were showed good stability in vitro. Herein rhenium was selected as a surrogate of radioactive 99mTc. However radioactive isotopes of rhenium (186-Re and 188 Re) is also used for radioactive therapy.

Subject Keywords

Magnetite., Nanoparticles

URI

http://etd.lib.metu.edu.tr/upload/12612750/index.pdf
https://hdl.handle.net/11511/20179

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins Aygar, Gülfem; Volkan, Mürvet; Özkan, Necati; Department of Micro and Nanotechnology (2011) The magnetic separation approach has several advantages compared with conventional separation methods; it can be performed directly in crude samples containing suspended solid materials without pretreatment, and can easily isolate some biomolecules from aqueous systems in the presence of magnetic gradient fields. This thesis focused on the development of new class of magnetic separation material particularly useful for the separation of histidine-tagged proteins from the complex matrixes through the use of ...
Recovery enhancement of magnetite fines in magnetic separation Arol, Ali İhsan (2004-01-23) Low intensity wet magnetic separation is not very effective for particles less than 10 mum. The magnitude of the fluid drag force exceeds the magnetic force exerted on such particles. Thus, fine magnetic particles are usually not picked up effectively by magnetic separators, resulting in the loss of valuable minerals. Magnetic separation applied to ultrafine magnetite particles after size enlargement by polymer flocculation were found to be effective in reducing loss of fine magnetite particles.
Direct epoxidation of propylene to propylene oxide on various catalytic systems: A combinatorial micro-reactor study Kalyoncu, Sule; Duzenli, Derya; Önal, Işık; Seubsai, Anusorn; Noon, Daniel; Senkan, Selim (2015-02-10) A combinatorial approach is used to investigate several bimetallic catalytic systems and the promoter effect on these catalysts to develop highly active and selective catalysts for direct epoxidation of propylene to propylene oxide (PO) using molecular oxygen. 2%Cu/5%Ru/c-SiO2 catalyst yielded the highest performance with high propylene conversion and PO selectivity among the bimetallic catalytic systems including silver, ruthenium, manganese and copper metals. On the other hand, the most effective catalyst...
Synthesis and Characterization of Polyhydroxybutyrate Coated Magnetic Nanoparticles: Toxicity Analyses on Different Cell Lines Yalcin, Serap; Khodadust, Rouhollah; Unsoy, Gozde; Garip, Immihan Ceren; Mumcuoglu, Zahide Didem; Gündüz, Ufuk (2015-01-01) In this study, polyhydroxybutyrate (PHB) coated magnetic nanoparticles were prepared which are targetable to tumor cells in the presence of a magnetic field. The structural properties, functional groups, size distribution, and magnetic properties of the synthesized PHB coated magnetic nanoparticles were characterized by X-ray diffraction, Fourier transform infrared spectrometer, transmission electron microscopy, dynamic light scattering, vibrating sample magnetometry, and thermogravimetric analysis. PHB coa...
Processing optimization of SiO2-capped aluminum-doped ZnO and amorphous In-Zn-Sn-Oxide thin films for transparent heater and near-infrared reflecting applications Can, Hilal Aybike; Öztürk, Tayfur; Akyıldız, Hasan; Department of Metallurgical and Materials Engineering (2022-2-09) In this study, AZO/SiO2 and amorphous In-Zn-Sn-oxide (a-ZITO) functional coatings were developed with combinatorial approach for transparent heater and heat reflecting applications. AZO/SiO2 and amorphous In-Zn-Sn-oxide (a-ZITO) coatings were obtained via magnetron sputtering. A series of optimization steps were carried out specific to each method and coating type. The samples were produced on 75x50 mm2 glass substrates. Further, optimum post-deposition annealing conditions were identified as 400 °C and Ar+...

Citation Formats

Ü. Zengin, “Preperation and characterization of silica coated magnetite nanoparticles and labeling with nonradioactive Re as a surrogate of Tc-99m for magneticly targeted imaging,” M.S. - Master of Science, Middle East Technical University, 2010.