Cellulose-based electrospun scaffolds for tissue engineering applications

Atila, Deniz Hazal
With the use of a scaffold as support material, adequate number of cells, and bioactive molecules, tissue engineering applications intend to promote the regeneration of tissues or to replace failing or malfunctioning tissues/organs. In this study, electrospun 2D and 3D cellulose-based scaffolds were aimed to be produced with pullulan (PULL). Cellulose acetate (CA) and PULL powders in various ratios (80/20, 50/50, and 80/20) were dissolved in DMAc/DMSO solvent system and electrospun as either 2D or 3D forms. Scaffolds were modified by crosslinking or sacrificial fiber removal and characterized. When the electrospun scaffolds were crosslinked with STMP, a crosslinker for PULL, weight loss was so high and this crosslinking demolished fiber morphology. Therefore, for plant origin cellulose based scaffolds instead of crosslinking, PULL fibers were used as sacrificial fibers to increase porosity and produce 3D scaffolds. After sacrificial fibers of PULL were removed, cell viability tests were conducted via Alamar Blue Assay using CA/PULL with 50/50 ratio having 82.90±6.77% porosity, 14.19 μm mean fiber diameter and 146.9±9.24 MPa compressive strength by seeding human osteogenic sarcoma cell line (Saos-2) and mouse fibroblastic cell line (L929) cell line. Cell culture studies showed that electrospun scaffolds were biocompatible. Bacterial cellulose (BC) pellicles synthesized by bacteria species Glucanoacetobacter xylinum, were modified in order to be electrospun with the addition of PULL or/and gelatin. Acetylation, powder size reduction by mixer milling and autoclave treatments, and sulfuric acid exposure of BC were performed. However, crystalline BC was not easily processable for electrospinning due to poor solubility in the solvent systems suitable for plant origin cellulose. FTIR-ATR showed that these modifications altered the physicochemical characteristics of BC however they could not be sufficient for complete dissolution.


Wet spun PCL scaffolds for tissue engineering
Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Polymeric scaffolds for bioactive agent delivery in bone tissue engineering
Uçar, Şeniz; Hasırcı, Nesrin; Yılgör, Pınar; Department of Chemistry (2012)
Tissue engineering is a multidisciplinary field that is rapidly emerging as a promising new approach in the restoration and reconstruction of tissues. In this approach, three dimensional (3D) scaffolds are of great importance. Scaffolds function both as supports for cell growth and depot for sustained release of required active agents (e.g. enzymes, genes, antibiotics, growth factors). Scaffolds should possess certain properties in accordance with usage conditions. Wet-spinning is a simple technique that ha...
Biodegradable poly (ester-urethane) scaffolds for bone tissue engineering
Kızıltay, Aysel; Hasırcı, Nesrin; Department of Biotechnology (2011)
During last decade, polyurethanes (PUs) which are able to degrade into harmless molecules upon implantation have received a significant level of attention as a biomaterial in tissue engineering applications. Many studies are focused especially on development of PUs based on amino acid derivatives; however, there are only few applications of amino acid based PUs in tissue engineering. In this study, a biocompatible and biodegradable thermoplastic poly(ester-urethane) (PEU) based on L-lysine diisocyanate (LDI...
Growth factor loaded silk fibroin/PEGDMA hydrogels for articular cartilage tissue engineering
Fathi Achachelouei, Milad; Tezcaner, Ayşen; Engin Vrana, Nihal; Department of Biomedical Engineering (2018)
The aim of this study was to develop bFGF and TGF-β1 loaded polymeric nanoparticles (PNPs) and dental pulp stem cells (DPSCs) containing dimethacrylated poly(ethylene glycol) (PEGDMA)/silk fibroin hydrogels as scaffolds for regeneration of the cartilage tissue. Poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) were prepared with double emulsion-solvent evaporation technique. The effect of different excipients (poly(2-ethyl-2-oxazoline) (PetOx), heparin and Kolliphor P 188) on the entrapment efficienc...
Fabrication and characterization of bilayered tissue scaffolds incorporating bioactive agents for skin tissue engineering applications
Aktürk, Ömer; Keskin, Dilek; Bilici, Temel; Department of Engineering Sciences (2015)
In this study, it was aimed to fabricate tissue scaffolds from different biological polymers (collagen, silk fibroin and sericin) for skin tissue engineering applications. For this purpose, bilayered scaffolds composed of epidermal (collagen/sericin films) and dermal (collagen sponges, collagen matrices or silk fibroin matrices) layers were produced with different biomaterial fabrication methods. Casting and solvent evaporation (film), lyophilization/freeze-drying (sponge) and dry/wet electro-spinning (micr...
Citation Formats
D. H. Atila, “Cellulose-based electrospun scaffolds for tissue engineering applications,” M.S. - Master of Science, Middle East Technical University, 2014.