The effects of streptozotocin induced-diabetes on rat testes and the recovery role of vitamin c

Download
2012
Güldağ, Damla
Type I Diabetes is a multisystem disease having both biochemical and structural consequences. It causes alterations in carbohydrate, protein, and fat metabolisms due to hyperglycemia. Type I diabetes is also correlated with increased formation of free radicals and decreased levels of antioxidant potential. Lower endogeneous antioxidant amounts and elevated lipid peroxidation levels in diabetes constitute the basis of risk factors for the development of diabetic complications. These complications lead to irreversible damages in nearly all vital organs and systems. Since the antioxidant capacity lowered in diabetic conditions, it becomes important to be able to use some common antioxidants, as a complementary treatment strategy for diabetes. The effect of type I diabetes and the recovery role of Vitamin C on the structure, composition and function of the macromolecular content of testicular tissue is still unknown. Therefore, in the current study, it was aimed to investigate the alterations in the macromolecules of rat testes due to Streptozotocin (STZ)-induced type I diabetes using Attenuated Total Reflectance (ATR)-Fourier Transform Infrared (FTIR) spectroscopic and FTIR microspectroscopic techniques. Furthermore it was iv aimed to gain useful information about the recovery role of Vitamin C, as an antioxidant, against the diabetic complications. The detailed spectral analysis revealed that, the macromolecular structure and composition of rat testes are highly affected due to the development of diabetes. The lipid and protein content of diabetic rat testes were shown to decrease considerably, indicating an increase in lipolysis and proteolysis processes. Diabetes was also shown to lead to a decrease in the content of fatty acids and nucleic acids. In addition to the compositional alterations, protein conformation, and protein secondary structural components were also found to alter in diabetic state. Besides, lipid peroxidation levels were found to increase, and the elevated levels of lipid peroxidation products end up with increased levels of unsaturation, and also end up with increased levels of disorderness in diabetic conditions. On the other hand, with the administration of Vitamin C, the diabetes-induced alterations were found to be partially recovered, indicating that after more confirmative researches, Vitamin C may have a chance to be used as a complementary therapy in the treatment of diabetes.

Suggestions

The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study
Bozkurt, Ozlem; Bilgin, Mehmet Dincer; Severcan, Feride (Hindawi Limited, 2007)
Diabetes mellitus (DM) is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by a defective insulin secretory response. Skeletal muscle takes role in determination of carbohydrate and lipid metabolism, therefore; it is one of the target tissues of diabetes. Herein this study, application of Fourier Transform Infrared (FTIR) spectroscopy in diabetic skeletal Extensor Digitorum Longus (EDL) muscle tissues will be presented which highlight the promise of this technique in me...
Early alterations in myocardia and vessels of the diabetic rat heart: an FTIR microspectroscopic study
Toyran, Neslihan; Lasch, Peter; Naumann, Dieter; Turan, Belma; Severcan, Feride (2006-08-01)
Diabetes mellitus is associated with a high incidence and poor prognosis of cardiovascular disease. The aim of the present study was to examine the effect of relatively short-term (5 weeks) Type I diabetes on the left ventricle, the right ventricle and the vessel (vein) on the left ventricle of the myocardium, at molecular level by FTIR (Fourier-transform infrared) microspectroscopy. The rats were categorized into two groups: control group (for the left ventricle myocardium, n = 8; for the right ventricle m...
Regulation of Glutathione S-Transferase Mu with type 1 diabetes and its regulation with antioxidants
SADİ, GÖKHAN; Kartal, Deniz Irtem; Güray, Nülüfer Tülün (2013-01-01)
Objective: Increased oxidative stress is now related with the pathogenesis and the chronic complications associated with the disease, diabetes mellitus. While roles of oxidative stress in diabetic complications are widely studied, the molecular mechanisms playing role in the regulations of detoxification enzymes in the presence of antioxidants have not been clearly established because of the complexity of the pathways.
Characterization of STING mediated innate immune sensing in the context of pathology
Şanlı, Hatice Asena; Gürsel, Mayda; Department of Molecular Biology and Genetics (2018)
Type I interferonopathies are a group of diseases characterized by excess type I interferon production. Here, we examined the intracellular signal transduction pathways of two interferonopathies, STING-associated vasculopathy with Onset in Infancy (SAVI) and Aicardi-Goutières syndrome (AGS) using in vitro cell line models. SAVI is characterized by constitutive STING activation. M155V and N154S gain-of-function mutations in STING can cause disease in humans. We expressed the M155V mutant STING in STING-knock...
Evaluation of an aldo-keto reductase gene signature with prognostic significance in colon cancer via activation of epithelial to mesenchymal transition and the p70S6K pathway
Canli, Secil Demirkol; Seza, Esin Gulce; Sheraj, Ilir; Gomceli, Ismail; Turhan, Nesrin; Carberry, Steven; Prehn, Jochen H. M.; GÜRE, ALİ OSMAY; Banerjee, Sreeparna (Oxford University Press (OUP), 2020-09-01)
AKR1B1 and AKR1B10, members of the aldo-keto reductase family of enzymes that participate in the polyol pathway of aldehyde metabolism, are aberrantly expressed in colon cancer. We previously showed that high expression of AKR1B1 (AKR1B1(HIGH)) was associated with enhanced motility, inflammation and poor clinical outcome in colon cancer patients. Using publicly available datasets and ex vivo gene expression analysis (n = 51, Ankara cohort), we have validated our previous in silico finding that AKR1B1(HIGH) ...
Citation Formats
D. Güldağ, “The effects of streptozotocin induced-diabetes on rat testes and the recovery role of vitamin c,” M.S. - Master of Science, Middle East Technical University, 2012.