Early alterations in myocardia and vessels of the diabetic rat heart: an FTIR microspectroscopic study

Toyran, Neslihan
Lasch, Peter
Naumann, Dieter
Turan, Belma
Severcan, Feride
Diabetes mellitus is associated with a high incidence and poor prognosis of cardiovascular disease. The aim of the present study was to examine the effect of relatively short-term (5 weeks) Type I diabetes on the left ventricle, the right ventricle and the vessel (vein) on the left ventricle of the myocardium, at molecular level by FTIR (Fourier-transform infrared) microspectroscopy. The rats were categorized into two groups: control group (for the left ventricle myocardium, n = 8; for the right ventricle myocardium, n = 9; for the vein, n = 9) and streptozotocin-induced diabetic group (for the left ventricle myocardium, n = 7; for the right ventricle myocardium, n = 9; for the vein, n = 8). Two adjacent cross-sections of 9 mu m thickness were taken from the ventricles of the hearts in two groups of rats by using a cryotome. The first sections were used for FTIR microspectroscopy measurements. The second serial sections were stained by haematoxylin/eosin for comparative purposes. Diabetes caused an increase in the content of lipids, an alteration in protein profile with a decrease in alpha-helix and an increase in beta-sheet structure as well as an increase in glycogen and glycolipid contents in both ventricles band the vein. Additionally, the collagen content was found to be increased in the vein of the diabetic group. The present study demonstrated that diabetes-induced alterations in the rat heart can be detected by correlating the IR spectral changes with biochemical profiles in detail. The present study for the first time demonstrated the diabetes-induced alterations at molecular level in both ventricle myocardia and the veins in relatively short-term diabetes


Fourier transform infrared study of the effect of diabetes on rat liver and heart tissues in the C-H region
Severcan, Feride; Toyran, N; Kaptan, N; Turan, B (2000-10-02)
Diabetes mellitus is characterized by hyperglycemia, a relative lack of insulin. The metabolic disturbances in diabetic patients are often associated with cardiac and liver dysfunctions. Generally, experimental diabetic models in animals have been used to study diabetes-related changes in organ function, but the complexity of intact tissues can cause contradictory results. For this reason, different techniques have been used to understand the mechanisms of these dysfunctions in diabetic organs. The purpose ...
Regulation of Glutathione S-Transferase Mu with type 1 diabetes and its regulation with antioxidants
SADİ, GÖKHAN; Kartal, Deniz Irtem; Güray, Nülüfer Tülün (2013-01-01)
Objective: Increased oxidative stress is now related with the pathogenesis and the chronic complications associated with the disease, diabetes mellitus. While roles of oxidative stress in diabetic complications are widely studied, the molecular mechanisms playing role in the regulations of detoxification enzymes in the presence of antioxidants have not been clearly established because of the complexity of the pathways.
The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study
Bozkurt, Ozlem; Bilgin, Mehmet Dincer; Severcan, Feride (Hindawi Limited, 2007)
Diabetes mellitus (DM) is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by a defective insulin secretory response. Skeletal muscle takes role in determination of carbohydrate and lipid metabolism, therefore; it is one of the target tissues of diabetes. Herein this study, application of Fourier Transform Infrared (FTIR) spectroscopy in diabetic skeletal Extensor Digitorum Longus (EDL) muscle tissues will be presented which highlight the promise of this technique in me...
The effects of streptozotocin induced-diabetes on rat testes and the recovery role of vitamin c
Güldağ, Damla; Severcan, Feride; Department of Biology (2012)
Type I Diabetes is a multisystem disease having both biochemical and structural consequences. It causes alterations in carbohydrate, protein, and fat metabolisms due to hyperglycemia. Type I diabetes is also correlated with increased formation of free radicals and decreased levels of antioxidant potential. Lower endogeneous antioxidant amounts and elevated lipid peroxidation levels in diabetes constitute the basis of risk factors for the development of diabetic complications. These complications lead to irr...
The effects of high cholesterol/high fat diet on endoplasmic reticulum stress and neuronal dysfunction in the hippocampus and cerebral cortex of APOE-/- MICE
Mengi, Naz; Yanık, Tülin; Department of Molecular Biology and Genetics (2019)
Hyperlipidemia is an obesity-associated lipid metabolism disorder with high serum total cholesterol (TC) levels and is known to be a risk factor for neurodegenerative diseases. High-fat diet (HFD) induced elevated inflammation levels accompanied by increased levels of apoptosis markers and decreased levels of synaptic proteins in the hippocampus points out a possible neuronal loss. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) pathway is activated by endoplasmic reticulum (ER) stress. The acti...
Citation Formats
N. Toyran, P. Lasch, D. Naumann, B. Turan, and F. Severcan, “Early alterations in myocardia and vessels of the diabetic rat heart: an FTIR microspectroscopic study,” BIOCHEMICAL JOURNAL, pp. 427–436, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/52027.