The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study

2007
Bozkurt, Ozlem
Bilgin, Mehmet Dincer
Severcan, Feride
Diabetes mellitus (DM) is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by a defective insulin secretory response. Skeletal muscle takes role in determination of carbohydrate and lipid metabolism, therefore; it is one of the target tissues of diabetes. Herein this study, application of Fourier Transform Infrared (FTIR) spectroscopy in diabetic skeletal Extensor Digitorum Longus (EDL) muscle tissues will be presented which highlight the promise of this technique in medical research. Type I DM was induced in rats by injection of streptozotocin (STZ) which is one of the most popular experimental models. In diabetes, a significant increase was observed in lipid order together with an increase in hydration of phospholipid molecules in membrane structure. There was a decrease in lipid and nucleic acid content in diabetic EDL muscles. A dramatic increase in the bandwidth of amide II band (1540 cm(-1)) and shifting of the position of this band to lower frequency values in diabetes was observed indicating structural changes occurring in proteins of diabetic EDL muscles.
Spectroscopy

Suggestions

The effects of high cholesterol/high fat diet on endoplasmic reticulum stress and neuronal dysfunction in the hippocampus and cerebral cortex of APOE-/- MICE
Mengi, Naz; Yanık, Tülin; Department of Molecular Biology and Genetics (2019)
Hyperlipidemia is an obesity-associated lipid metabolism disorder with high serum total cholesterol (TC) levels and is known to be a risk factor for neurodegenerative diseases. High-fat diet (HFD) induced elevated inflammation levels accompanied by increased levels of apoptosis markers and decreased levels of synaptic proteins in the hippocampus points out a possible neuronal loss. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) pathway is activated by endoplasmic reticulum (ER) stress. The acti...
Fourier transform infrared study of the effect of diabetes on rat liver and heart tissues in the C-H region
Severcan, Feride; Toyran, N; Kaptan, N; Turan, B (2000-10-02)
Diabetes mellitus is characterized by hyperglycemia, a relative lack of insulin. The metabolic disturbances in diabetic patients are often associated with cardiac and liver dysfunctions. Generally, experimental diabetic models in animals have been used to study diabetes-related changes in organ function, but the complexity of intact tissues can cause contradictory results. For this reason, different techniques have been used to understand the mechanisms of these dysfunctions in diabetic organs. The purpose ...
The effects of streptozotocin induced-diabetes on rat testes and the recovery role of vitamin c
Güldağ, Damla; Severcan, Feride; Department of Biology (2012)
Type I Diabetes is a multisystem disease having both biochemical and structural consequences. It causes alterations in carbohydrate, protein, and fat metabolisms due to hyperglycemia. Type I diabetes is also correlated with increased formation of free radicals and decreased levels of antioxidant potential. Lower endogeneous antioxidant amounts and elevated lipid peroxidation levels in diabetes constitute the basis of risk factors for the development of diabetic complications. These complications lead to irr...
Early alterations in myocardia and vessels of the diabetic rat heart: an FTIR microspectroscopic study
Toyran, Neslihan; Lasch, Peter; Naumann, Dieter; Turan, Belma; Severcan, Feride (2006-08-01)
Diabetes mellitus is associated with a high incidence and poor prognosis of cardiovascular disease. The aim of the present study was to examine the effect of relatively short-term (5 weeks) Type I diabetes on the left ventricle, the right ventricle and the vessel (vein) on the left ventricle of the myocardium, at molecular level by FTIR (Fourier-transform infrared) microspectroscopy. The rats were categorized into two groups: control group (for the left ventricle myocardium, n = 8; for the right ventricle m...
The effects of antioxidants on some rat tissues and membranes
Görgülü, Güvenç; Güray, Tülin; Department of Biochemistry (2004)
High blood glucose levels induce metabolic disorders that initiate a sequence of events including renal, arterial, cardiac and retinal disorders. Diabetes mellitus increases oxidative stress in tissues of animals including humans. The resulting oxidative stress might play role in the development of diabetic complications. In the present study, 36 male Wistar rats (250-300g) were divided into 5 groups as Control (n=6), Diabetic (n=7), Diabetic + Vit C (n=7), Diabetic + a-Lipoic acid (n=6) and Diabetic + Comb...
Citation Formats
O. Bozkurt, M. D. Bilgin, and F. Severcan, “The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study,” Spectroscopy, pp. 151–160, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51735.